FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192893
Sequences flagged as poor quality0
Sequence length125
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC67903.520086265442499No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA61913.2095514093305613No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG59053.061282680035045No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT56322.9197534384347796No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA47152.444360344854401No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC46782.425178726029457No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC43992.280538951646768No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA42352.1955177222605275No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC36131.8730591571493003No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA35181.8238090547609296No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA34501.7885563498934645No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA33561.7398246696354973No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT32701.6952403664207618No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG26291.3629317808318602No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC25181.3053869243570269No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA25161.3043500800962191No Hit
AACAGAGAATCTCCATTTTAGCACGACTTTGTCATATTTTTCCCCTCTGT24261.257692088359868No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC23791.2333262482308844No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA22581.1705971704520122No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG21751.1275681336284882No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT21691.124457600846065No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA21631.1213470680636415No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA21341.1063128262819284No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT21331.1057944041515244No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG20791.0777996091097137No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA20401.0575811460239615No Hit
CAGCCAGACTGCCTTCCGTAGCAGAGACCTGTGGGAAGCGAAATGTAGAA17900.9279756134229858No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG17610.9129413716412725No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT17420.9030913511635985No Hit
CCTCCTGGCCCCTGTCGAACACTTCAATGCCTGGCCGTATTGTAGAACCA15820.820143810298974No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC15240.7900753267355476No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT14290.7408252243471769No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG14270.7397883800863692No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT12560.6511381957873018No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA12420.6438802859616471No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT12360.6407697531792237No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG12220.633511843353569No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA12180.6314381548319534No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA10790.559377478705811No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG10680.5536748352713681No Hit
TCCAGGAAGAGGAAAGGAAAAACAAGTCTCATCTTCACTTAGCCATTGGT10390.5386405934896549No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC10210.5293089951423847Illumina Single End PCR Primer 1 (100% over 21bp)
CACACACAGGTAACGGCTGACGACATGCCTTTAACTGTCAAACTGTCATG9900.5132379090998637No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT9600.49768524518774654No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT9560.49561155666613094No Hit
TGCCCTGACTTTCAACTCTGTACACTCTACAAGCAGTCACAGCACATGTA9210.4774667821019944No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA9020.46761676162432025No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGTTGCAACA8850.45880358540745386No Hit
CAGCCAGACTGCCTTCCGTAGAGACCTGTGGGAAGCGAAATGTAGAACCA8810.45672989688583826No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT8370.4339193231480665No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC7980.4137008600623143No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA7890.4090350608886792No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT7650.3965929297589855No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCTCTTGCCGTC7600.394000819106966No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6150.3188296101984001No Hit
CTTGGGCCTGTGTTATCTCCTGCCACCTGCTTGCCTCTGACCCCTGTAGA5770.29912956924305184No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA5680.2944637700694167No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC5370.2783926840268957No Hit
AGAATGGTCCTGCACCAGTAATATGCACATTAAAACAAGATTTACCTCTA4780.24780577833306547No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG4650.24106629063781476No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA4170.21618202837842743No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT3980.20633200790075326No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGAC3970.20581358577034936No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC3880.20114778659671423No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA3850.19959252020550255No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA3320.1721161472940957No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC2900.15034241781713176No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2850.14775030716511225No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTTCTTGTTGGCTTTCG2750.14256608586107325No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG2740.14204766373066932No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC2610.1353081760354186No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2390.12390288916653273No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2380.12338446703612883No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG2380.12338446703612883No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC2290.1187186678624937No Hit
CCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT2240.11612655721047419No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA2210.1145712908192625No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA2210.1145712908192625No Hit
TCCCTGCTTCTGTCTCCTACGGCCACCTGAAGTCCAAAAAGGGTCAGTCT2180.11301602442805078No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCACTTCT2090.10835022525441566No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG2080.10783180312401176No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT2060.10679495886320395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)