FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371612
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG140523.781363357480383No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA125643.3809457175763966No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT97142.6140167701796497No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA91562.463860155215655No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT87572.35649010258011No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC84372.270378782170651No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC78002.098963434980571No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTAGAGTATCAT76552.059944242920035No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT76472.057791459909798No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG74452.0034336889013273No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT68411.840898571628473No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC65091.7515580767036587No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA62611.6848218033863278No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA62181.6732505947063065No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG61941.6667922456755973No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA59871.6110889852857282No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT59371.5976340914717502No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC59081.5898302530596427No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG57681.5521565503805044No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA56921.5317051117832579No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT53711.4453246934975188No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT50101.3481803601605975No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA49311.3269216279345124No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA48481.304586504203309No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG48241.2981281551725994No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT47951.290324316760492No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG46871.2612617461222997No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC45351.2203588689278064No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA44481.1969473536914848No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC41701.122138144085767No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT41561.118370773817853No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG41491.116487088683896No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG39511.0632057091805431No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG39091.0519035983768017No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC38391.0330667470372323No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG38121.0258011043776842No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC37651.013153504192545No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT37421.006964253038115No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG34000.9149327793505054No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG32610.8775281745476464No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG28820.7755400794376931No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC28120.7567032280981238No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA26910.7241423850682971No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC22290.5998191662271402No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT20880.5618763656717221No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT20860.561338169919163No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA17260.46446293445852127No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACTAGAGTATCATCTCG13980.37619883103882545No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG8610.2316932714767015No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG8390.2257731181985512No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA8200.2206602585492395No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC7210.19401956879756305No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA7060.18998310065336962No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC6210.167109781169607No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACTAGAGTATCATCT6020.16199692152029535No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACTAGAGTA5760.1550003767370268No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG5450.1466583425723604No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC4460.12001765282068394No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3790.1019880951099534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)