FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243897
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA207068.489649319179819No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCCCTCAACCTGCGTCATGTGCTG203278.334255853905542No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG165596.789341402313272No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA134955.533073387536542No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC86273.5371488784199885No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC84663.4711374063641616No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA80103.2841732370631864No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT69782.8610438012767685No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG68132.793392292648126No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT64992.6646494216821037No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG57252.34730234484229No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTACGACATAT56712.32516185110928No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT46501.9065425158981044No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT41951.719988355740333No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA36631.5018634915558615No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG35131.4403621200752776No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC32521.3333497336990616No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG26611.0910343300655605No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT26101.070123863762162No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC16570.6793851502888515No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCTACGACATATCTCG14620.5994333673640921No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC11570.47438057868690475No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTACGAC10580.4337896735097192No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG10520.43132961865049596No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATCT9000.3690082288835041No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATCTACGACATAT6270.2570757327888412No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG5990.24559547677913215No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG5980.24518546763592827No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA5560.22796508362136475No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT5460.2238649921893258No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGATGGGAGGCTG5450.22345498304612194No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG4530.18573414187136372No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG4430.1816340504393248No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGAACCCA4320.17712394986408198No Hit
AGCTGCTCACCATCGCTACACTGACGACATGGTTCTACAGGTCTTGGCCA3850.157853520133499No Hit
TGTGGTCTGCCAGCTAAGGGTGAAGATATATTCCTCCAATTCAGGACCCA3650.1496533372694211No Hit
TGTGGTCTGCCAGCTAAAGGTGAGGATATATTCCTCCAATTCAGGACCCA3640.1492433281262172No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCTA3470.14227317269175102No Hit
AGCTGCTCACCATCGCTATCTGAGTAGCCCTCAACCTGCGTCATGTGCTG3370.1381730812597121No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT3310.13571302640048874No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTACGACATAT3220.1320229441116537No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT3090.12669282525000308No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT2970.12177271553155636No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAA2900.11890265152912909No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTA2890.11849264238592522No Hit
GGGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATCTACGACATATCTCG2860.11726261495631353No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTA2830.11603258752670184No Hit
GACCCAAAACCCAAAATGGCAGGGAAGGGAGAGATGGGGGTGGGAGGCTG2750.11275251438107069No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCCCTCAACCTGCATCATGTGCTG2680.10988245037864344No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2660.10906243209223565No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT2510.10291229494417725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)