FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243897
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC242789.954201978704125No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT183747.533507997228337No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT138185.6655063407914No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT84483.4637572417864924No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG79013.2394822404539623No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC75673.102539186623862No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG74333.04759796143454No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG71602.935665465339877No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG57172.3440222716966588No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT52342.1459878555291785No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA52332.1455778463859745No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT45281.8565214004272295No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA37961.5563947076019795No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA36701.5047335555582888No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG34851.4288818640655687No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA32631.3378598342743042No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT31981.311209239966051No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT17160.7035756897378812No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA14780.6059935136553545No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC14660.6010734039369078Illumina Single End PCR Primer 1 (100% over 21bp)
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT12900.5289117947330226No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT9690.39729885976457274No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG9550.3915587317597183No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT9440.3870486311844754No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT9370.3841785671820481No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG9140.3747483568883586No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA8950.36695818316748463No Hit
CAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT6400.2624058516504918No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG6280.2574857419320451No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6280.2574857419320451No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT5980.24518546763592827No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG5940.24354543106311272No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT5580.22878510190777254No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5160.21156471789320902No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTTTAGCATCTTGTTCTGTTT4800.19680438873786885No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC4660.19106426073301433No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG4650.19065425158981048No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA4570.1873741784441793No Hit
CATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT4300.17630393157767418No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGCGATGGTGAG3910.16031357499272234No Hit
CTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTTG3580.14678327326699384No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3280.13448299897087707No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTTCACACCCC3200.1312029258252459No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT3180.1303829075388381No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGTTGTGGGTTGATTCCACACCCC3100.12710283439320696No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACATCCC2930.12013267895874077No Hit
CCAACTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2910.11931266067233298Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT2710.11111247780825513No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT2600.1066023772330123No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC2570.10537234980340061No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT2570.10537234980340061No Hit
CCAACTGGCCAAGACCTGTACGGTAGCGATGGTGAGCAGCTTGTAGAACC2520.10332230408738115No Hit
CACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT2490.10209227665776946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)