FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280221
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT240118.568594073963052No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA238298.50364533707324No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG198897.09761224176632No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA159615.695861480759829No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC104753.7381209830812114No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA96353.4383575820513093No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC94123.3587775363017047No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG90053.2135350312788837No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT89103.1796332180671683No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGTACTAGAAT79942.852748366467895No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT78942.8170622472976685No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG68452.4427148572019943No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT63242.2567901763251146No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA45921.6387065922967945No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG44581.590887192608691No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG41111.4670563590880057No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT35731.2750650379521877No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC25230.9003607866648109No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATAGTACTAGAATCTCG21480.7665378397764622No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC17050.6084483318523594No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC15900.5674092948065991No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATAGTACTA14370.5128095324761528No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG13240.47248421781379696No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATAG10750.38362578107993334No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG9200.3283122963660825No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG8700.31046923678096927No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG8090.2887007040871312No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATAGTACTAGAAT8040.2869163981286199No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA7970.2844183697867041No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT7270.2594380863675456No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT5590.19948540616156535No Hit
GCCTTCTTTAAGCAATGCCATCTTTATCATTTCTTCCAGTGTTCTAATTG5230.18663840326028383No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGTACTAGAAT5170.18449723611007027No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT4700.16772476010006387No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATAGT4340.15487775719878238No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT4330.15452089600708013No Hit
TGTGGTCTGCCAGCTAAGGGTGAAGATATATTCCTCCAATTCAGGACCCA4090.1459562274062258No Hit
GGGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATAGTACTAGAATCTCG4070.14524250502282127No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATAGT3970.14167389310579864No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG3790.1352503916551579No Hit
AGCTGCTCACCATCGCTACACTGACGACATGGTTCTACAGGTCTTGGCCA3760.1341798080800511No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG3110.11098383061940396No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA2930.10456032916876323No Hit
GCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCTC2840.10134857844344286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)