FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398694
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA268896.744270041686105No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT189864.762048087004069No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA127683.202456018901714No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG121433.0456941915353632No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA114992.8841668046170748No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT110382.7685392807516545No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA109912.7567507913337046No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC105852.654918308276523No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC103392.5932168530251274No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG101402.543303887191681No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT94882.3797699488831032No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATCATATCTCAT80912.029375912353835No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC70251.7620029395977868No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT68901.728142384886655No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT68111.7083276899075481No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG66321.6634311025498252No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT60261.511434834735411No Hit
TGACAGTTTGACAGTTAAAGGCATGTTGGTCTCAGCCGTTACCTGTGTGT58431.4655349716825437No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG56601.419635108629676No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA53541.3428845179511104No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC53451.3406271476370348No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA52101.306766592925903No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG51171.28344043301379No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG46751.1725784687003065No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCACACCAGGACTA45101.1311933462755897No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT44091.1058606349731874No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG41761.0474198257310119No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC41181.0328723281514143No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG35620.8934170065263085No Hit
ATACGGCCAGGCATTGAAGTACACTGGACGACAGGGGCCAGGAGGAGACC29460.7389125494740327No Hit
GGAATCCTATGGCTTTCCAACCAGCAACAGAGGAGGGGGAGACCAAGTCT29100.7298830682177309No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG28590.7170913031046366No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT25840.6481160990634421No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAATCATATCTCATCTCG24440.6130014497333794No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC23450.5881703762785495No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC20960.5257164642557952No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT20710.5194459911611412No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAATC19910.4993804772582482No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA18790.47128875779419804No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGATATGGACGACGTGGTGGAT18610.46677401716604716No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA16930.42463643796997197No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC16320.4093364836190161No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA15050.37748248029817355No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAATCATA11940.2994777950006772No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAATCATATCTCATCT11110.2786598243264258No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC9210.23100422880705504No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA8300.2081797067425143No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAATC8000.20065513902892945No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGATGGGT7850.19689285517213703No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA7550.18936828745855216No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAATCATATC7220.18109126297360884No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTCTTGGCCAGTTGG7090.17783061696438873No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA6870.17231260064109316No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA6850.17181096279352084No Hit
ACTTCTACCGTGCCCTGATGGACGAAGAAGACATGGACGACGTGGTGGAT6020.15099299211926942No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT5590.14020777839646445No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA5150.12917174574987333No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA5130.12867010790230102No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCAAGGTCTTGGCCAGTTGG4920.12340291050279162No Hit
AGCTGCTCACCATCGCTATACGGTAGCAGGTCTTGGCCAGTTGGAGACCA4790.1201422644935715No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAATCATATCTCAT4370.10960786969455272No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAAT4310.10810295615183575No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAATC4290.10760131830426342No Hit
TGTTCATTCATGATCCCACTGCCAGCGAGATCTCCTCCATCCTGGAGAAA4100.10283575875232635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)