FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179148
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA173449.681380757809185No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG144318.055350883068748No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCCCTCAACCTGCGTCATGTGCTG138117.709268314466251No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA99255.5401120860964115No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC73954.12787192712171No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGAGACGACAAT62673.498224931341684No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC61643.4407305691383656No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA55313.0873914305490433No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG42892.394109897961462No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT42522.3734565833835712No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT42382.3656418157054504No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG42102.3500122803492083No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT41862.336615535758144No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT31751.7722776698595577No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC17931.0008484604907675No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA15520.8663228168888294No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG15490.8646482238149462No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGAGACGACAATCTCG14990.8367383392502289No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT14210.7931989193292697No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG12080.6743028110835734No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGAGACGA8250.4605130953178378No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT7810.43595239690088644No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTACGA6810.3801326277714515No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC6800.3795744300801572No Hit
GCCTTCTTTAAGCAATGCCATCTTTATCATTTCTTCCAGTGTTCTAATTG5270.29417018331212186No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG4420.24672337955210216No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC4280.2389086118739813No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTACGAGACGACAAT4070.22718646035679996No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG3980.2221626811351508No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA3750.20932413423538082No Hit
AGCTGCTCACCATCGCTATCTGAGCAACCCTCAACCTGCGTCATGTGCTG3520.19648558733561078No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGAGACGACAAT2990.1669011096970103No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGAACCCA2970.1657847143144216No Hit
GGGGCCAGTTGGAGACCAAGTCTCTGCTACCGTACGAGACGACAATCTCG2770.15462076048853463No Hit
TGTGGTCTGCCAGCTAAGGGTGAAGATATATTCCTCCAATTCAGGACCCA2730.15238796972335722No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGAG2570.14345680666264765No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCCCTCAACCTGCGTCATGTACTG2320.12950186438028893No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTACGAGACGACAATCTCG2290.12782727130640587No Hit
TGTGGTCTGCCAGCTAAAGGTAAAGATATATTCCTCCAATTCAGGACCCA2280.12726907361511153No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGAG2140.11945430593699065No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTTCAATTCAGGACCCA1990.1110813405675754No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG1970.1099649451849867No Hit
AGCTGCTCACCATCGCTATCTAAGCAGCCCTCAACCTGCGTCATGTGCTG1940.10829035211110367No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG1910.10661575903722063No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCCCTCAACCTACGTCATGTGCTG1900.10605756134592628No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG1810.10103378212427713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)