FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179148
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC1827110.198830017639047No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT149348.336124321789805No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT113466.333311005425682No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG69763.8939870944693777No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG63403.5389733628061713No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT61783.4485453368164865No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC52122.909326367026146No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG49952.788197468015272No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG44632.4912362962466785No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT33021.8431687766539397No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA31381.7516243552816664No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT31151.7387858083818966No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA25441.4200549266528233No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA18091.009779623551477No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT17780.9924754951213522No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC15470.8635318284323577Illumina Single End PCR Primer 1 (100% over 21bp)
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG15380.8585080492107084No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA14970.8356219438676402No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT9460.528055015964454No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT9210.5141000736820952No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT9040.5046107129300913No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT7860.43874338535735813No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG7460.41641547770558424No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA6660.37175966240203634No Hit
CAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT6370.35557192935450016No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA6050.337709603233081No Hit
CAATGGAAGCACAGACTGCAATTTGTACCTGAATGACAAGGTAGCGCTGG5530.3086833232857749No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5370.2997521602250653No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4180.2333266349610378No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4160.2322102395784491No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT3970.22160448344385647No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT3780.21099872730926386No Hit
CTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTTG3750.20932413423538082No Hit
CATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT3650.2037421573224373No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG3310.18476343581842947No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA3250.18141424967066336No Hit
CCAACTGGCCAAGACCTGCCCTGTGTAGCTGTGGGTTGATTCCACACCCC3170.17694866814030857No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3000.16745930738830464No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA2840.15852814432759507No Hit
CCAACTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2830.1579699466363007Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT2650.14792238819300244No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2350.13117645745417197Illumina Single End PCR Primer 1 (95% over 22bp)
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT2300.12838546899770023No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCTC2280.12726907361511153No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT2230.12447808515863978No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTAGCATCTTGTTCTGTTTG2210.12336168977605108No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTTTTAGCATCTTGTTCTGTT2150.120012503628285No Hit
AAGGGTGCAGTTATGCCTCAAATTCACTTTTATCACCTTTCCTTGCCTCT2060.11498872440663585No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACATCCC2010.11219773595016411No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC1980.11052314287628105No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTTTAGCATCTTGTTCTGTTT1940.10829035211110367No Hit
CACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT1940.10829035211110367No Hit
GTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGA1840.10270837519816017No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT1830.10215017750686584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)