FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177886
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA1906810.719224671980932No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT173839.771988801816894No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG161759.092902195788314No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA105285.9183971757192815No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC78484.411814308039981No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC65973.7085549171941583No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA60133.380254769908818No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG45132.537018090237568No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT45022.5308343545866454No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATGCTGCGCTAT43722.4577538423484704No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG42812.406597483781748No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT41822.350943862923445No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT36312.041194922590873No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG17981.0107597000326052No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG16120.90619835175337No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT15580.8758418312852052No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA13490.7583508539176776No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC13420.7544157494125451No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAATGCTGCGCTATCTCG11240.6318653519669901No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAATG8390.47165038282945254No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT8370.47052606725655755No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG7990.44916407137155256No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAATGCTGCG7270.40868871074733254No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC5540.3114354136919151No Hit
GCCTTCTTTAAGCAATGCCATCTTTATCATTTCTTCCAGTGTTCTAATTG4760.26758710634901006No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC4270.24004137481308252No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAATGCTGCGCTAT4130.23217116580281755No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA3760.21137132770426004No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG3580.20125248754820504No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATGCTGCGCTAT3450.19394443632438754No Hit
GGGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAATGCTGCGCTATCTCG3400.19113364739215002No Hit
TGTGGTCTGCCAGCTAAGGGTGAAGATATATTCCTCCAATTCAGGACCCA2970.16696086257490753No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAATGC2950.16583654700201253No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAATGC2630.1478474978356925No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG2140.12030176629976502No Hit
GCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCTC2130.11973960851331751No Hit
NGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA1910.10737213721147253No Hit
AGCTGCTCACCATCGCTACGGTGCAGGTCTTGGCCAGTTGGAGACCAAGT1790.10062624377410252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)