FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177886
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC174909.832139684966776No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT158678.919757597562484No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT125347.046085695332967No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG74524.189199824606771No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT65193.664706609851253No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC57833.250958479025893No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG47012.6427037540896983No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG46772.6292119672149576No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG43952.4706834714367627No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT34231.924266103009793No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA32621.8337586993917452No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA27811.5633608041104978No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT22731.2777846485951678No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT18091.0169434356835276No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG16350.9191279808416627No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA14020.7881452165993952No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA13730.7718426407924176No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT13560.7622859584228101No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC11280.6341139831127801Illumina Single End PCR Primer 1 (100% over 21bp)
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT10970.6166870917329076No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT9800.5509146307185501No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT8540.48008274962616504No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT8510.4783962762668226No Hit
CAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT8280.4654666471785301No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA8220.46209370045984505No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC7970.44803975579865757No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCTGC6810.3828294525707476No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG6440.36202961447219006No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5120.28782478666112005No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA5020.28220320879664507No Hit
CTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTTG4990.28051673543730254No Hit
CAATGGAAGCACAGACTGCAATTTGTACCTGAATGACAAGGTAGCGCTGG4880.27433299978638004No Hit
CATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT4680.2630898440574301No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4310.24229000595887254No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4130.23217116580281755No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT3520.19787954082952003No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3440.19338227853794004No Hit
CCAACTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC3400.19113364739215002Illumina Single End PCR Primer 1 (95% over 22bp)
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA3240.18213912280899003No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG3000.16864733593425002No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTTTAGCATCTTGTTCTGTTT2630.1478474978356925No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT2630.1478474978356925No Hit
CTCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT2530.1422259199712175No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT2400.13491786874740003No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA2290.12873413309647752No Hit
GTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTGA2170.12198823965910752No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTTTTAGCATCTTGTTCTGTT2120.11917745072687001No Hit
CCAACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCC2090.11749097736752752No Hit
CTGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA1950.10962076835726252No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTAGCATCTTGTTCTGTTTG1940.109058610570815No Hit
CCAACTGGCCAAGACCTGCACCGTAGCGATGGTGAGCAGCTTGTAGAACC1780.10006408598765501No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)