FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637219
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA375085.88620238881766No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT292734.593868042227241No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA258754.060613384095578No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG223323.504603597821157No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA211253.3151867725224764No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA195433.0669204778890773No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC191283.0017937318253223No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT183622.8815838824642706No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC173122.7168053683270585No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA169592.6614084011933103No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT138122.1675436545363524No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA135992.1341171559542325No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT131942.0705597290727367No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATCTAGATCAAT131372.061614609733859No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC108181.69768949136796No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT94401.4814373080526475No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC93511.4674703673305411No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG87841.3784899696964465No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT82651.2970423041371961No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA81701.2821337719057342No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT79741.2513751159334545No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG79521.2479226137324844No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG73391.151723347859998No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG70561.1073116150020637No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG60760.953518335140666No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA57600.9039278489812764No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA51830.8133781321649229No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC48170.7559410500942376No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC41460.6506397329646479No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT39840.6252167622120495No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT38960.611406753408169No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA36610.5745277526250787No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATAGCGCGGACGCGGGTGCCGGGC33970.5330977262134369No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG33740.5294882920942408No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAATC33290.5224263557740745No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAACCCCTCAGGGCAACTG31130.48852906143727665No Hit
GGGGTCAGAGGCAAGCAGAGAGGAGGGGAGATAACACAGGCCCAAGAGAC30650.48099632936243264No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC25060.39327138707414566No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC24230.3802460378613946No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA23960.3760088760692949No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAATCTAGATCAATCTCG23410.3673776205668695No Hit
GGAAACCGTAGCTGCCCTGTTAAAAATCATCTGGACCTGGGTCTTAGACC23230.36455284603880295No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC20990.3294000963561978No Hit
GCGTGGACAACCCCCACACCAAGTTCATGCTGCAGAAGGAGGGGAGATAA19700.30915587890505464No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA18420.28906859337213736No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG18330.2876562061081041No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAATCTAGATCAATCT15840.24858015846985101No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAATCTAG15580.24449992859597722No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC14010.21986161743450838No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA13060.20495308520304634No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT13000.20401149369369087No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGGAGACCA11700.18361034432432177No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAATCTAGAT10600.16634783331947103No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA10090.1583443054899493No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA9770.15332248410671998No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAAT9590.1504977095786535No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT9050.14202338599445402No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC8880.13935554338461345No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGGCA8800.13810008803880613No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGCGGAT8680.13621690502009515No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC8560.13433372200138413No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG8420.13213667514622132No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA8160.1280564452723475No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG8020.1258593984171847No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7810.12256382813444044No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAATC7270.11408950455024096No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAATC6930.10875381933055982No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAATCTAGATCAAT6870.10781222782120432No Hit
GCGTGGACAACCCCCACGCCAAGTTCATGCTGCAGAAGGAGGGGAGATAA6850.10749836398475249No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGCGTGCAGGGT6820.10702756823007474No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC6670.104673589456686No Hit
GGAAACCGTAGCTGCCCTGTTAATCATCTGGACCTGGGTCTTAGACCAAG6650.10435972562023417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)