FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637219
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC302974.754566326490579No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA263084.1285649046874No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA202993.18556100806787No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC202603.179440663257059No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT191473.0047754382716145No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC175912.760589373512089No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA169002.652149418017981No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG165212.592672221010359No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA154702.427736774954921No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA147122.308782380939677No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT137672.1604817182161864No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC136242.13804045390988No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG132012.0716582525003178No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA130512.0481184647664303No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT120601.8925989338045475No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG119811.8802013122647003No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA94081.4764154866694181No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA93421.4660579800665077No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT85901.348045177560619No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC82201.2899803678170298No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGCTGATTCCACACCCC77971.2235981664074673No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG77581.2174778215966566No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGT71141.1164136662591666No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG71071.1153151428315853No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG63430.9954191573069855No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT60290.9461425349840479No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT57180.8973367084157879No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT54200.850570996784465No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA41490.6511105287193256No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA39110.6137607321815577No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG38850.6096805023076839No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC35980.5646410417768459No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC33660.5282328367484334No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC33650.5280759048302075No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA31330.491667699801795No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA30210.4740913249604924No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC29870.46875563974081125No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTCTGCTTGCCTCTGACCCCTGTA29780.46734325247677805No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA29390.46122290766596724No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC26690.41885128974497No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC23890.37491035264171346No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC23800.3734979653776802Illumina Single End PCR Primer 1 (100% over 21bp)
AAGACCCAGGTCCAGATGATTTTTAACAGGGCAGCTACGGTTTCCTGTAG21230.33316646239361974No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC19480.3057033767040845No Hit
GGCTCCTTATCTCCCCTCCTTCTGCAGCATGAACTTGGTGTGGGGGTTGT19360.3038201936853735No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC18470.2898532529632669No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG17710.27792642717809735No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC14540.22817900910048192No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT13210.2073070639764351No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGAGAGATTTCA12800.20087285532917254No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA12780.20055899149272072No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT12640.19836194463755788No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA12390.19443864668191No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG12100.1898876210533584No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG11840.1858073911794846No Hit
CCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA11680.18329648048786995No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG9820.15410714369784956No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC9790.1536363479431718No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT9020.1415525902397763No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG9000.14123872640332444No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG8720.1368446326929988No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT8190.12852724102702526No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA7670.12036678127927761No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGGGAGACAGAGATTTCA7400.11612961948717787No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA7300.11456030030491872No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA7210.11314791304088546No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC7080.11110779810394855No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA6910.10843995549410798No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6890.10812609165765615No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT6800.10671370439362292No Hit
GGCTCCTTATCTCCCCTCCTTCTGCAGCATGAACTTGGCGTGGGGGTTGT6660.10451665753846008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)