FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559366
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG233664.1772292202243255No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA205233.6689752326741347No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT160542.8700350039151465No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA150722.694479106702946No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT140992.52053217392548No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC135752.4268546890586844No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC124582.227164325325458No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT122982.1985605131523904No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACATCTGGAGTAT117622.102737742372615No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG104191.8626444939449305No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT100931.8043642266423057No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC100581.7981071427294473No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA99481.7784420218604633No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA98651.7636037942956848No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG97991.7518047217742945No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC94771.6942395497759963No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA91691.6391772113428418No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT89021.5914445997790356No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG88621.5842936467357687No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT87161.5581926681278448No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG83621.494906733694933No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA82931.4825713396952978No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA80641.4416321335225952No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT80311.4357325972619002No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC79771.4260788106534898No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG77521.3858546997851138No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA74131.3252503727434273No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA73931.321674896221794No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT72991.3048701565701168No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG72651.2987918464833401No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC70651.2630370812670058No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC66471.1883096219648674No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT65101.1638176077916784No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG65001.1620298695308617No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT63441.134141152662121No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC61891.106431209619462No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG60581.0830118384027632No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG58691.0492235852733274No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG52150.9323055030159144No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC48940.874919104843698No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG47350.8464940664967123No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA40260.7197434238048076No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA39650.7088382204138256No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC37890.6773740270234516No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT36200.6471612504156492No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG28710.5132596546804775No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT20090.3591566165980771No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACATCTGGAGTATCTCG10840.1937908274725314No Hit
TCAAATCATCCATTGCTTGGTACGGGCAGAAAGTCAGTCCCATGGAGACC9900.17698608782085432No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA9340.16697475356028074No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACATCTGGAGTATCT9120.16304172938648398No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC8170.14605821590872523No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC7200.12871715477880313No Hit
AGCTGCTCACCATCGCTAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCT6340.11334260573577944No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACATCTGGAGTAT6070.10851571243157432No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT5980.10690674799683929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)