FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708723
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC271553.831539261460401No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG221243.1216709490167527No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC220173.1065733721072974No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA185702.6202056374634375No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC182662.57731158717863No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG134881.9031412836891142No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG132851.874498217216035No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA131931.8615171230508958No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG126851.7898389074433876No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT126501.78490044770665No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA115121.6243299568378617No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA114121.610220071875754No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC112411.5860921685905496No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC111781.5772029410644217No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT110201.5549093228242912No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC103471.459949797029305No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG93941.3254825933404164No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC90771.2807542580105344No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG89431.2618470121613097No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT88251.2451973479060225No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT87921.2405410858685268No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA86481.2202228515230915No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT83361.1762000104413148No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT80801.1400787049383185No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT78861.1127055281118294No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT77561.094362677661089No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA75951.0716457628720952No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC71761.012525344880863No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT71731.012102048332No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT71361.00688139089602No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT70430.9937591978812597No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT69850.9855754646032371No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA61470.8673346286207728No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG60120.8482862839219272No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT59540.8401025506439046No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT58370.8235939852382382No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC57200.8070854198325721No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT52610.7423210478564969No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA51340.7244014939546197No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT47490.6700784368505044No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT45060.6357914163925822No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC43790.6178718624907051No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG43730.6170252693929786No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG40870.57667099840135No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC40120.5660885846797691No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT36620.5167039873123914No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA34400.48538004269651186No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC33800.47691411171924714No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC31950.4508108245393475No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT31380.44276819011094604No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA31270.44121610276511414No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA31190.44008731196814554No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA21250.299835055444793No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC20690.2919335198660125Illumina Single End PCR Primer 1 (100% over 21bp)
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT18380.25933968560354326No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG13430.18949575504110916No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT13200.18625048149982432No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC11640.164239060958936No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT11070.1561964265305345No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC11000.15520873458318693No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA9310.1313630289972246No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG7400.10441314871959849No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT7240.10215556712566123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)