FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385380
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTTGTTCACAT145023.763039078312315No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG125673.2609372567336132No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA123663.2087809434843533No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA99932.593025066168457No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT91912.3849187814624524No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT87672.2748975037625203No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC87522.2710052415797395No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT81042.1028595152836163No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC80162.08002491047797No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG78552.0382479630494577No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA67411.749182624941616No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA66581.7276454408635633No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG65701.704810836057917No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA62831.6303388862940476No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT58501.5179822512844465No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG58421.5159063781202968No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC57121.4821734392028647No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC54441.4126316882038508No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT52561.3638486688463336No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT50381.307281125123255No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA50031.2981991800301No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC49421.282370647153459No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA49281.278737869116197No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT47231.2255436192848617No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG45891.1907727437853546No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT45811.188696870621205No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG45801.1884373864756863No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC40341.0467590430224714No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT39281.0192537235974881No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG38450.9977165395194354No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT37580.9751414188593077No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA36360.9434843531060252No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG35280.9154600653900048No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG33570.8710882765063054No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG31840.8261975193315687No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC31830.8259380351860501No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG31360.8137422803466708No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC29620.7685920390264155No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA28250.7330427110903524No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA27240.7068348123929629No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC25660.6658363174010068No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT22690.5887695261819503No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT21940.5693082152680471No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA20750.5384296019513208No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC20550.5332399190409466No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCTTGTTCACATCTCG20380.5288286885671285No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA15860.41154185479267213No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT13420.34822772328610724No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12330.31994395142456794No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC11010.2856920442160984No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC10900.2828377186153926No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTTGTTCACAT9760.2532565260262598No Hit
TTTCGCTTCCCACAGGTCTCTGCGGAAGGCAGTCTGGCTGAGACCAAGTC9360.24287716020551142No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA8110.21044164201567284No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG7860.2039545383777051No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA7660.19876485546733094No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG7290.18916394208313872No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA7140.1852716799003581No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTTGTTC6540.16970263116923556No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC6130.1590637812029685No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG6100.15828532876641238No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATCTTGTTCACATCT5910.1533551300015569No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG5410.14038092272562147No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5050.13103949348694793No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGAAGAGATGGGGGTGGGAGGCTG4700.12195754839379315No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG4600.11936270693860605No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTT4600.11936270693860605No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATCT4550.11806528621101252No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATCTTGTTCACAT4530.11754631791997508No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG4260.11054024599096994No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTT4030.10457211064403965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)