FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326713
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGGAGCATGTAT130213.9854551242221765No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA112663.4482864165184735No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA91232.792359042952071No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT80652.468527423151206No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG78452.401190035290913No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT70222.1492869888862702No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC62991.9279918460544883No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA57371.7559754279750115No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG55201.6895562772219042No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA54161.6577240575061294No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG52051.5931413809673933No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT50391.5423322610364447No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA48181.474688794140423No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG45631.3966386400296285No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT44811.3715401590998828No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC44191.3525632588847092No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC43911.3439930458843083No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT43721.3381775442054646No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA41971.2846137129529587No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC41511.2705340773094427No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC41431.268085445023614No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT40451.2380896995222106No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC39681.214521613771108No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA38451.1768738923764894No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT36611.1205553498024259No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG33991.0403626424415313No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA32330.9895535225105827No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT32200.9855744950461108No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT31810.9736374126526951No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC31310.9583334608662649No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT31260.9568030656876219No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG30190.924052608864661No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC30160.9231343717574753No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC29910.9154823958642601No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG28590.8750799631480841No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG25790.7893778331440745No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA24570.7520361907851846No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG23670.72448907756961No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA23230.7110215999975513No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG22770.6969419643540354No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG20350.6228708377077129No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA19390.5934872502777667No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATGGAGCATGTATCTCG16940.5184978865242583No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG16890.5169674913456153No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC15940.48788998295139774No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT15570.4765650586294393No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT14250.4361626259132633No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC13840.4236133854483905No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA11030.33760517640865223No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT10870.33270791183699455No Hit
TTTCGCTTCCCACAGGTCTCTACGGAAGGCAGTCTGGCTGAGACCAAGTC9860.30179392922840537No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC9600.2938358742994616No Hit
TGTTCATTCATGATCCCACTGCTACCGTAGCCAAGTGTGAGTGACTGGTG9530.29169332104936135No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA9500.29077508394217555No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC9180.2809805547988602No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA8970.27455289504855945No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG8180.25037265122599955No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA8060.24669970279725628No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGGAGCATGTAT7990.24455714954715607No Hit
AGCTGCTCACCATCGCTAGCAGGTCTTGGCCAGTTGGAGACCAAGTCTCT7780.23812948979685536No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG7450.22802888161781135No Hit
GACCCAAAACCCAAAATGGCAGGAGAGAGAGAGATGGGGGTGGGAGGCTG6950.21272492983138105No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG6820.20874590236690918No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGGAGCAT6230.19068723925892145No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA6200.18976900215173564No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATGGAGCATGTATCT6080.18609605372299234No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGG5450.16681307447209018No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA5140.1573246243645034No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGGA5010.15334559690003152No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC4890.14967264847128825No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATGGAGCATGTAT4870.14906049039983105No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGA4860.14875441136410245No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCTACTTGATAAGAG4780.1463057790782736No Hit
GAGAAAGCCCCCCTACTGCTGTCTAACCTTCCACTCGGATAAGATGCTAG4540.13895988222078706No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGG4400.13467477572058656No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG4260.1303896692203861No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4220.12916535307747168No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4190.12824711597028585No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG4160.12732887886310004No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC4130.1264106417559142No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGGA4090.12518632561299978No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG3850.11784042875551326No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT3810.11661611261259883No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT3620.1108006109337553No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTTACAGCAGGGTTTTC3590.1098823738265695No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTA3540.10835197864792648No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATGG3370.10314863504054016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)