FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450831
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGAAGGAGATAAT164743.6541409086775314No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA146733.254656401179156No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG134252.977834266055351No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA123192.73250952130621No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT113552.518682166931733No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT93202.0672935090976443No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC89131.9770157775308266No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA78861.7492142288351955No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA78051.7312474075651407No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG75381.6720234411564423No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA68541.520303617098203No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT67561.4985659814875196No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG64321.4266986964073012No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT61351.3608203517504343No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT59691.323999458777236No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG59661.323334020952419No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC57841.282964126246864No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA57511.2756443101738788No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC56971.2636664293271758No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC56641.2563466132541905No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC55071.2215220337554427No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC55061.2213002211471706No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC52421.1627416925632885No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG52181.157418189964754No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC50641.123259048290823No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT49701.1024086631132286No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA47381.05094813799406No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA45601.0114654937215941No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC45060.9994876128748911No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT42610.9451435238481826No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA42180.9356055816924745No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG38580.855753042714454No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG37570.8333499692789537No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC33520.7435158629286805No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG33330.7393014233715073No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG32850.7286544181744379No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT31650.7020369051817643No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAAATGGAGGTGGGAGGCTG28850.6399293748655261No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT26020.5771564067244711No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGAAGGAGATAATCTCG24100.5345683859361934No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG20780.4609265999897966No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC20490.45449403434990054No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG20380.4520540956589054No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA20280.4498359695761826No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT20060.44495609219419247No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT19520.43297821134748943No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG18650.41368051442780107No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA17100.3792995601455978No Hit
TGTTCATTCATGATCCCACTGCTACAAGTGTGAGTGACTGGTGGAGACCA16790.37242336928915715No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA16430.36443811539135507No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA14850.3293917232843349No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG14620.32429003329407247No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA14050.31164671462255256No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG13760.3052141489826565No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC13360.2963416446517653No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG13180.29234901770286426No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT11580.2568590003792996No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGAAGGAGATAAT11580.2568590003792996No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG10560.23423411433552707No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAAAGATGGAGGTGGGAGGCTG10330.22913242434526465No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGACGCTGAGG9790.21715454349856156No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG9750.21626729306547243No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA9710.2153800426323833No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG9620.2133837291579328No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA9460.2098347274255763No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT8680.19253334398033853No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGAAGGAGA8640.19164609354724943No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACTGGGTGCGGAAGGCAGTCT8210.18210815139154138No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGAAGGAGATAATCT8100.1796682127005463No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAA8010.1776718992260958No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT7150.15859601491467978No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA6910.15327251231614508No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG6850.1519416366665114No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGTTTCATCCTCTCAGGG6780.15038894840860545No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAA6680.14817082232588266No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCAGGC6480.14373457016043706No Hit
TCCACAAACAGAACAAGATGCTACCGTAAAATCCTGTTTGAAGGAAAAGG6220.1379674423453578No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGAAG6160.13663656669572413No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGAAG6020.1335311901799122No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG5950.13197850192200625No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGAAGGAGATAAT5940.13175668931373397No Hit
AGCTGCTCACCATCGCTACCGAGGTCTTGGCCAGTTGGAGACCAAGTCTC5900.13086943888064487No Hit
AGCTGCTCACCATCGCTACACTGACGACATGGTTCTACAGGTCTTGGCCA5880.1304258136641003No Hit
TCATACCAGGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGAAGGA5610.12443687324074876No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC5540.1228841849828428No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGACAGCGCCT5460.12110968411666455No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAGAAGGAGATAAT5420.12022243368357544No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAAGGAGATAA4940.10957542848650603No Hit
GCAACAGATAACTCAGATTGCCTTCTGACGACATGGTTCTACATCATACC4930.10935361587823375No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCATTGTA4890.10846636544514464No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC4880.10824455283687236No Hit
AGAAAACGGCATTTTGAGTGTACACTGAGGCATAACTGCACCCTTAGACC4820.10691367718723868No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGAAGGCTG4730.10491736371278815No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGTATCTCTACGGGCCATTCT4710.10447373849624361No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG4630.10269923763006536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)