FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450831
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG166903.7020524320643435No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC134522.9838232064787027No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC129622.8751350284252855No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA124172.754247156916893No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA83181.8450372756088202No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG78871.7494360414434678No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG74711.6571619964021993No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA73281.6254427934192635No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA69741.5469211300908767No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG69161.5340559988110845No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC69141.53361237359454No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT65471.4522071463586135No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC59761.325552147035142No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC55481.2306163506946062No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA54991.2197475328892644No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC54481.2084350898673781No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA52001.153425563015853No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG50691.1243681113321844No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT50631.1230372356825506No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT50391.117713733084016No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA50321.1161610448261101No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT49361.0948670344319713No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT49081.0886562814003473No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG48431.0742384618626493No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT43160.957343217303158No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC42710.9473616499309053No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG41400.9183041982472369No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT40500.8983410635027316No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA38210.8475459762083797No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT30630.6794120191379919No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC29710.6590052591769422No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT29520.6547908196197688No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA29330.6505763800625954No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT28470.6315004957511795No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT27910.6190789896879318No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT27900.6188571770796595No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG26380.5851416606222731No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT25670.5693929654349412No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC24490.5432190776588123Illumina Single End PCR Primer 1 (100% over 21bp)
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC23420.5194851285736783No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT22930.5086163107683367No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT22360.4959729920968168No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT20890.4633665386807917No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA19510.4327563987392171No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT17890.3968227561991079No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC17590.3901683779509395No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG17530.3888375023013058No Hit
CCACCAGTCACTCACACTTGTAGCAGTGGGATCATGAATGAACATGTAGA16890.3746414953718799No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA16400.3637726775665382No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC14110.31297759027218625No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC13200.2927926429194088No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC12990.28813457814569093No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG12970.28769095292914637No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG11610.2575244382041164No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCGTGAAAACAACG10960.24310661866641822No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC9670.2144927921992942No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT9480.21027835264212086No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT9280.20584210047667528No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC9150.20295853656913565No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA9140.20273672396086337No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA8660.19208971876379397No Hit
TTCTCTTCCGCACCCAGCAGTTTAGCCAGCCCAAAATCTGTGATCTTGAC8400.1863225909487147No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT8260.1832172144329028No Hit
CAGCCAGACTGCCTTCCGCACCCAGTACGGTAGCAGAGACCTGTGGGAAG8220.1823299639998137No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7730.171461146194472No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG7700.17079570836965513No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7420.1645849553380313No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG7160.15881782752295207No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTAAAATAGAAAATCAA7060.15659970144022928No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC6910.15327251231614508No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6810.15105438623342227No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT6670.14794900971761038No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA6600.1463963214597044No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAACGATGGTGAGCGGCTGGGG6480.14373457016043706No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA6170.1368583793039964No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGCGATGGTGAG5970.1324221271385508No Hit
GGCCTTTTCCTTCAAACAGGATTTTACGGTAGCATCTTGTTCTGTTTGTG5890.13064762627237259No Hit
CCAACTGGCCAAGACCTCGGTAGCGATGGTGAGCAGCTTGTAGAACCATG5850.12976037583928346No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5710.12665499932347155No Hit
TCCCTGCTTCTGTCTCCTACAGCCACTTGAAGTCCAAAAAGGGTCAGTCT5680.12598956149865473No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT5540.1228841849828428No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5500.12199693454975369No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT5440.12066605890012No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA5310.11778249499258037No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA5300.11756068238430809No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAACGATGGTGAGCAGCTGGGG4930.10935361587823375No Hit
GTTTCCTCTGGTCCTGGTATGATGTAGAACCATGTCGTCAGTGTAGATCT4910.10890999066168919No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG4850.10757911501205551No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTC4760.105582801537605No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTTACCGTAGCTTC4730.10491736371278815No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCATCGTAGCTCC4700.10425192588797133No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA4690.10403011327969904No Hit
GTTTCCTCTGGTCCTGGTATGATGTAGAACCATGTCGTCAGAAGGCAATC4680.10380830067142675No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG4620.10247742502179308No Hit
TCCAGGAAGAGGAAAGGAAAAACATCGAAAAATAACTTACCTTTTTGTCT4610.10225561241352082No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATATATGGAAGGATGAGAATTT4610.10225561241352082No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATATCTCACCAATG4580.10159017458870397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)