FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408955
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTTCTTCGTAAT174214.259881894095927No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA134633.292049247472216No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA118232.8910271301243413No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG107802.6359868445183454No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT106022.5924612732452226No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG99662.4369429399322664No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC88742.1699208959420964No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC87202.1322639410204056No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA77141.8862711056228678No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC73281.7918841926373317No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA68721.6803804819601178No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG67991.6625301072245111No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC65201.5943074421391108No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC57061.3952635375530316No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA56781.3884168184763603No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT55331.352960594686457No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG53061.2974532650291597No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT44741.0940078981795063No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC44731.0937633724981966No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG42831.0473034930493574No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA41611.0174713599295766No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA40200.9829932388649117No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG40140.9815260847770537No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG39660.9697888520741891No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC38890.9509603746133438No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT38320.937022410778692No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA36850.9010771356261691No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT36790.8996099815383111No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG31470.7695223190815615No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG29590.7235514909953418No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT28080.6866281131175802No Hit
CTGGTGTTGTTGGGCAGTGCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTG26760.6543507231847024No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT26670.6521499920529154No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTTCTTCGTAATCTCG25930.634055091635999No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA25870.632587937548141No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA25190.6159601912190827No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTTACAGCAGGGTCTTC24930.6096025235050312No Hit
TCCACAAACAGAACAAGATGCTTCTACCGTAATCCTGTTTGAAGGAAAAG24420.5971317137582375No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG23820.5824601728796567No Hit
TCAAATCATCCATTGCTTGGTCGGTAGCAGAAAGTCAGTCCCATGGAGAC23360.5712119915394115No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG22560.551649937034637No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG21810.5333105109364111No Hit
TCTTAAATGGCTACGACCCAGCTACCTGTTAAAGAATCATCTGGAAGACC21640.5291535743541466No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA21550.5269528432223595No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT19460.4758469758286364No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA19330.47266814197161056No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA19230.47022288515851374No Hit
AGCTGCTCACCATCGCTACCGAGGTCTTGGCCAGTTGGAGACCAAGTCTC18570.4540841901920749No Hit
ACTTCTACCGTGCCCTGATCAAGCTGTGTCTACTCATTTGAACAGACCAA17920.4381900209069457No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT16220.3966206550843002No Hit
CAGAGGGGAAAAATATGACAAGTCTCTGCTAGTGCTAAAATGGAGATTCT15700.3839053196561969No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGAAGGCTG14660.35847464879999025No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA14510.354806763580345No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTTCTTCGTAAT14390.35187245540462886No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGATGCCGGGC14040.3433140565587901No Hit
CAGCCTCTGGCATTCTGGGAACTTCATCTGGACCTGGGTCTTCAGTGAAC13660.3340220806690222No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG12940.3164162316147253No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA12860.3144600261642479No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG12450.30443447323055106No Hit
ACTTCTACCGTGCCCTGTGGCACAAGCTGTGTCTACTCATTTGAACAGAC12180.2978322798351897No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTACAGGGT11770.28780672690149284No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG11140.272401608978983No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTTCTTCG10650.2604198505948087No Hit
GACCCAAAACCCAAAATGGCCAGTAGGAGACAGAAGCAGGGAAGACCAAG10280.25137240038635056No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA10170.2486826178919441No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTT9880.2415913731339634No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTTCTTCGTAATCT9570.23401107701336332No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG8940.2186059590908535No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTTC8690.21249281705811152No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT8570.20955850888239538No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGTTCTCTTTAACAATT8540.20882493183846632No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT8190.20026653299262753No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCTTTTACT8130.19879937890476948No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTT8080.19757675049822107No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT7840.19170813414678878No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA7820.1912190827841694No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTTC7610.18608404347666613No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC7450.18217163257571126No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC7360.17997090144392414No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT7350.17972637576261447No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG7280.17801469599344671No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCTCCTCAGGGCAACTG7070.17287965668594346No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC6820.16676651465320144No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGTTCTTCGTAAT6720.16432125784010465No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTTCTTCGTAA6570.16065337262045945No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT6500.1589416928512917No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC6220.15209497377462067No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAGTTCTT6200.15160592241200133No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTT5930.14500372901663997No Hit
ATACGGCCAGGCATTGAAGTACACTGAAGACAGGGGCCAGGAGGAGACCA5890.14402562629140125No Hit
CCTCAGAAAAAGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGCTACCG5810.1420694208409238No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG5050.12348546906138817No Hit
GGAATCCTATGGCTTTCCAACCTGTCTACAGCAACAGAGGAGGGGGAGAC4780.11688327566602681No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA4470.10930297954542675No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC4300.10514604296316221No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGTT4190.10245626046875574No Hit
GGAATCCTATGGCTTTCCAACCTTCTACAGCAACAGAGGAGGGGGAGACC4090.10001100365565893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)