FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408955
Sequences flagged as poor quality0
Sequence length125
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG173964.253768752063185No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC136743.3436441662285583No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA126073.08273526427113No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC104782.5621400887628223No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA72301.7679206758689832No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA69621.7023877932779892No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC69301.6945629714760793No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA65621.6045775207541173No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA62631.5314643420425231No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG61731.5094570307246518No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC57931.4165372718269735No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA57631.4092015013876833No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC54901.3424459903901407No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA54551.333887591544302No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG50771.241456884009243No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC49121.201110146593146No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT48991.1979313127361202No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT48131.1769021041434877No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC45361.1091684904207064No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG43251.0575735716643642No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG42791.0463253903241188No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA41331.0106246408529056No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT41131.005734127226712No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTCTGGCCCCTCCT40620.9932633174799184No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG39680.9702779034368084No Hit
CACACACAGGTAACGGCTGAGGGGACTCAAAGTACATGAACTTGTCTTCC38970.9529165800638213No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG38690.9460698609871502No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA32190.7871281681358585No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT31150.7616974972796519No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT30140.7370004034673742No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG27990.684427381985793No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC26090.6379675025369539Illumina Single End PCR Primer 1 (100% over 21bp)
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG25640.6269638468780184No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT25530.6242740643836119No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG24310.5944419312638309No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT24220.5922412001320438No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA24170.5910185717254954No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT23870.5836828012862051No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT23730.5802594417478696No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGAAGCATCTTGTTCTGTTTGT23630.5778141849347728No Hit
CCATGGGACTGACTTTCTGCTACCGACCAAGCAATGGATGATTTGATGTA23410.5724346199459598No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC21820.5335550366177209No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC21670.5298871513980755No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG21230.5191280214204497No Hit
TCCAGATGATTCTTTAACAGGTAGCTGGGTCGTAGCCATTTAAGATGTAG20660.5051900575857979No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA20620.5042119548605593No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT19280.4714455135650622No Hit
CCAACTGGCCAAGACCTCGGTAGCGATGGTGAGCAGCTTGTAGAACCATG18320.4479710481593329No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA17950.43892359795087477No Hit
GTTCAAATGAGTAGACACAGCTTGATCAGGGCACGGTAGAAGTTGTAGAA17480.4274308909293198No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC17230.42131774889657786No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA16540.40444547688621No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTGTCATATTTTTCCC14970.36605494492059026No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG14290.3494271985915321No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC12850.3142155004829382No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAATAGTGGTAATCTACTGGGACG12390.3029673191426929No Hit
GTTCAAATGAGTAGACACAGCTTGTGCCACAGGGCACGGTAGAAGTTGTA11920.291474612121138No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT11660.28511694440708635No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA11510.2814490591874412No Hit
GCTGCCCCCACCATGAGCGTTGCTCAGATAGCGATGGTGAGCAGCTGGGG10970.26824467239671845No Hit
CCTCCTGGCCCCTGTCATTTTCTGTCCCTTCCCAGAAAACCTACCAGGGC10920.26702204399017004No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA10680.2611534276387377No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA9770.23890159063955693No Hit
TCCCTGCTTCTGTCTCCTACTGGCCATTTTGGGTTTTGGGTCTGTAGAAC9480.2318103458815762No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG9390.2296096147497891No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC9100.2225183699918084No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA8930.21836143340954384No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC8350.20417894389358243No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT8220.2010001100365566No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT7770.189996454377621No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT7730.1890183516523823No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC7570.18510594075142742No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC7540.18437236370749838No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT7220.17654754190558863No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6900.1687227201036789No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTTTA6820.16676651465320144No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG6690.1635876807961756No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT6650.1626095780709369No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG6490.15869716716998203No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6150.15038329400545294No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT6000.14671540878580774No Hit
CCTCCTGGCCCCTGTCTTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA5990.14647088310449805No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA5940.14524825469794966No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5870.14353657492878188No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG5830.14255847220354317No Hit
ACAAGTCAACAACCCCCACTTTTTCTGAGGTGTAGAACCATGTCGTCAGT5510.13473365040163343No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC5310.12984313677543985No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC5130.1254416745118656No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACGGAGATTTCA5010.12250736633614946No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC4920.12030663520436234No Hit
CCCCCTCCTCTGTTGCTGTAGACAGGTTGGAAAGCCATAGGATTCCTGTA4890.11957305816043329No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT4880.11932853247912362No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC4600.1124818134024526No Hit
CCCCCTCCTCTGTTGCTGTAGAAGGTTGGAAAGCCATAGGATTCCTGTAG4090.10001100365565893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)