FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428508
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACCATATGATCAT180854.220457961111578No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA153913.591764914540686No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG120482.8116161191856395No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT116752.7245699030123123No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA114902.6813968467333167No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC101652.372184416626994No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG87572.043602453163068No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT84541.9728919880142262No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA81251.8961139581991466No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC73721.7203879507500444No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC72701.696584427828652No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC64511.505456140842178No Hit
GACCCAAAACCCAAAATGGCAATTGTGATGTAGAACGTAGGAGACAGAAG62951.4690507528447543No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG62361.4552820484098312No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT60661.4156095102075108No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT59341.3848049511327676No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG53851.25668598952645No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT49151.1470030897906225No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC48141.1234329347410084No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA48021.120632520279668No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA47691.1129313805109822No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA45371.0587900342584036No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC44011.0270520036965471No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG43971.0261185322094337No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG42991.0032484807751547No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG42871.0004480663138144No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA42530.9925135586733503No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG38580.9003332493208995No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT37140.8667282757848161No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA37030.8641612291952542No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT35120.8195879656855882No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG33170.7740812306888086No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACCATATGATCATCTCG27720.6468957405696043No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA27640.6450287975953775No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG26240.6123572955464076No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT25890.604189420034165No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA24910.5813193685998861No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA23930.5584493171656072No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG22560.5264779187319722No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG22340.5213438255528484No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC21610.5043079709130285No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG21460.5008074528363531No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA21430.5001073492210181No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC21420.4998739813492397No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG21290.4968401990161211No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT20450.47723729778673907No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG20070.46836931865916154No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC20020.46720247930026976No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA19140.4466661065837744No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG19020.44386569212243415No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT18510.43196393066173794No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA18050.42122900855993356No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG18010.42029553707282014No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT15980.37292185910181375No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC15480.3612534655128959No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG15030.35075191128286987No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA13600.3173803056185649No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT13340.31131274095232764No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA12530.29240994333828074No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG12330.2877425859027136No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACCATATGATCAT12280.28657574654382184No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT12160.2837753320824815No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA11850.2765409280573525No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC11500.26837305254511No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC11020.25717139469974887No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTAGTGGAT10710.24993699067461986No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT10620.24783667982861465No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA10300.2403689079317072No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC10210.238268597085702No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCATACCAGGAC9750.22753367498389762No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA9180.2142317062925313No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACCATATGA9110.2125981311900828No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACCATATGATCATCT9080.21189802757474774No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT8880.20723067013918062No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG8410.19626238016559783No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACCA8360.19509554080670605No Hit
TGTTCATTCATGATCCCACTGCCAGCGAGATCTCCTCCATCCTGGAGAAA8170.19066155124291728No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA8060.18809450465335537No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC6860.16009036003995258No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT6590.15378942750193697No Hit
GACCCAAAACCCAAAATGGCAAGGGAGGGAGAGATGGGGGTGGGAGGCTG6430.15005554155348325No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTACCATATGATCAT6350.1481885985792564No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATCATACCAGGACCA5900.13768704434923035No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACCAT5870.13698694073389528No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC5540.1292858009652095No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT5510.12858569734987443No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACCA5210.12158466119652377No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG5190.12111792545296703No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA5160.12041782183763196No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC5090.11878424673518347No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTACCA4680.10921616399227085No Hit
TCACAATTGCCAGTTAACGTCTTCTTTCTCTCTCTGTCATAGGGACTCTG4650.10851606037693579No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACTA4590.10711585314626565No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA4550.10618238165915223No Hit
TGTGCTGTGACTGCTTGTAGTACGGTAACAGAGTTGAAAGTCAGGGCAAG4380.10221512783892017No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTA4350.1015150242235851No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA4310.10058155273647168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)