FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449377
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGGTCTGGTGTAT172733.843765924824813No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA146993.270972924738026No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT117172.6073875610011195No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA111342.477652394314796No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG99002.2030500003337954No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG85241.8968483033177042No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT84991.891285045741104No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC79901.7780171214815177No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA72651.6166826517601034No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA69631.5494785002347695No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA68321.520327030533383No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG64651.4386584093088877No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT64551.4364331062782474No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG63511.4132899547595894No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC62761.396600182029788No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT57891.2882279244376103No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC57721.284444909285522No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA56771.2633045304944401No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC54081.2034438789702187No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC53031.1800781971484966No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG52351.1649461365401432No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA51631.148923954719534No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT51471.1453634698705095No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC50781.1300088789590923No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA48451.0781593183451756No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT46311.0305378334894755No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC45731.0176310759117622No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT45601.0147381819719299No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG43610.9704546516621901No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG43200.9613309092365654No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT42880.9542099395385167No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT42410.9437510152945078No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG40960.9114841213502249No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG39730.8841128940733505No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA39260.8736539698293416No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG38970.8672005910404849No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC38340.8531811819474516No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC32840.7307895152622409No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC32150.7154349243508235No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGGTCTGGTGTATCTCG27000.6008318182728534No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT24810.5520976819018331No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA24170.5378557425057358No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA23290.5182730758361019No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC22900.5095943940166052No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG22460.49980306068178837No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC21060.46864881825282556No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA20530.4568547121904325No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG20440.45485193946285635No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT19460.43304396976258247No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG17720.394323697029443No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATAGGGGTGGGAGGCTG15890.3536006515687274No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC15600.34714727277987084No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGGTCTGGTGTAT15100.33602075762666983No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG13670.304198924288515No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA13310.2961878333782103No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG13280.2955202424690182No Hit
TCAAATCATCCATTGCTTGGAGACCAAGTCTCTGCTACCGTAGGTCTGGT12320.2741573333748723No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT12100.2692616667074639No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA11980.2665913030706957No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG10900.24255803033978152No Hit
GACCCAAAACCCAAAATGGCAGAGGAGGGAGAGATGGGGGTGGGAGGCTG10410.23165404548964452No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGGAAGGCAGTCTGGCTGAGACCA10080.2243105454885319No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG10030.2231978939732118No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG9820.21852475760886736No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGGTC9380.20873342427405053No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA9210.2049504091219622No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGGTCTGGT9180.20428281821277014No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGGTCTGGTGTATCT8610.19159859093812098No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGGTC8400.18692545457377657No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA8300.18470015154313638No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG8150.1813621969971761No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT7990.17780171214815177No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC7930.17646653032976767No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCTTCCCT7480.16645266669188677No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG7150.15910916669077413No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGGT7030.1564388030540059No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG6900.15354590911417362No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGGTCTGGTGTAT6890.1533233788111096No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT6780.15087554547740537No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT6490.14442216668854882No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACTTGTTAAAGAATCATC6440.14330951517322874No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGGT6430.1430869848701647No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAACCAGCCCCTCAGGGCAACTG6130.1364110757782441No Hit
TCAAATCATCCATTGCTTGGAGACCAAGTCTCTGCTACCGTAAGCAGAAA5790.12884504547406744No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTCAGAC5670.12617468183729919No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGGT5330.11860865153312253No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA5260.1170509394116744No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGGT5050.11237780304732996No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4820.10725960607685751No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCGTGGACAAC4680.10414418183396124No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTA4560.101473818197193No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACATGGTGGAT4540.10102875759106496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)