FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383428
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGTTACGATCAT154214.021876336626433No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA125723.2788424423881404No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT108092.819042949393367No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA97702.5480663905609395No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC94162.455741364741229No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG82982.1641611984518607No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG72711.8963143015116268No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG72331.8864037055196803No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA70431.8368507255599484No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC68551.7878193559155826No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA67711.7659117226702277No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC60871.5875209948151934No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT59641.5554419604202092No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC57021.487111009107316No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC51831.3517531322699439No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTTGTGGGGGTTGTTGACTT50621.3201957081903253No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT48861.2742940004381527No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA48231.2578632755041363No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA47851.24795267951219No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG45221.1793609230416142No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC41811.0904263642717797No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA38891.0142712582284026No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG37990.9907987940369508No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG36530.9527212410152623No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA35460.9248150891432029No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG35060.9143828828358909No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA33710.8791741865487133No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT32910.8583097739340894No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT32470.8468343469960462No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG29630.7727656822141314No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGATGGGAGGCTG29050.7576389830685292No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT28520.7438163097113408No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA28350.7393826220307333No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT28340.7391218168730505No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG27550.7185182094161093No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA26810.6992186277475824No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG26380.688004005967222No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA25200.6572289973606518No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGAAGAGATGGGGGTGGGAGGCTG24340.6347997537999311No Hit
ATACGGCCAGGCATTGAAGTACATGGTTCGACAGGGGCCAGGAGGAGACC24020.6264539887540816No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT22160.5779442294250812No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATGTTACGATCATCTCG22150.5776834242673983No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT21430.5589054529142369No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT20490.5343897680920539No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCC19870.5182198483157203No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGGCTACCTGTTAAAGAAT19740.5148293812658439No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT19180.5002242924356072No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA18690.4874448397091501No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTCTCAGGAGGCTTGCAAATATCT18580.4845759829746393No Hit
GGATGCAGAGCTTCTTCCCAACATTTAGCAGGCCTGGCTCATTCTACAGA16730.4363270288033216No Hit
GCAACAGATAACTCAGATTGCCTTCATACCAGGACCAGAGGAAACAGACC16220.42302596576149887No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT16210.42276516060381614No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG16170.42172193997308494No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAAACATTATGACA16150.4212003296577193No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG12560.3275712780495947No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG12540.3270496677342291No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACACTATGACA11700.30514203448887406No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGTTACGATCAT11510.3001867364929009No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA11110.28975453018558894No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA10310.2688901175709651No Hit
ACTTCTACCGTGCCCTGATGGATGAAAAAGACATGGACGACGTGGTGGAT10050.2621091834712123No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC9600.2503729513754864No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA9530.2485473152717068No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA9150.23863671927976046No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATGTTACGATCATCT8900.2321165903376905No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT8890.2318557851800077No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA8880.2315949800223249No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC8740.22794370781476578No Hit
CAGCCTCTGGCATTCTGGTAGGTAGCAGAGCAGTCAGAGGACCAGGAGAC8720.22742209749940012No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGT8210.21412103445757744No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC7930.20681849004245906No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC7840.20447124362331387No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGTTACGA7550.19690789405051273No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG7490.19534306310441596No Hit
CAGAGGGGAAAAATATGACAAATACACTGACGACATACACTGACGACATG7150.1864756877432008No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGTCTTTGCTACCGTAGGA7060.18412844132405562No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC7030.18334602585100723No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGT6980.18204200006259325No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATGTTACGATCAT6890.17969475364344806No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT6730.17552187112052328No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCACTCATGGTGGGGGCAGCGCCT5900.15387504303285102No Hit
GGGGTCAGAGGCAAGCAGGAGGGGAGATAACACAGGCCCAAGAGACCAAG5850.15257101724443703No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATGTTAC5670.14787652440614665No Hit
GGGGTCAGAGGCAAGCAGACCAAGGGAGATAACACAGGCCCAAGAGACCA5490.14318203156785628No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATGT5440.1418780057794423No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGTT5310.1384875387295659No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC5170.13483626652200675No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGTT4980.12988096852603356No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTACACTGACGACAGG4900.1277945272645712No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG4850.1264905014761572No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAAGAA4770.1244040602146948No Hit
CTGGTGTTGTTGGGCAGTTCGTGGTGAGGCTCCCCTTTCTTGCGGAGATA4680.12205681379554963No Hit
ATACGGCCAGGCATTGAAGTACACTGAACGACAGGGGCCAGGAGGAGACC4580.11944876221872164No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG4510.11762312611494205No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCGACAGG4360.11371104874970006No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGTTACGATCA4150.10823414043836131No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAGAGACCAAG4090.10666930949226451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)