FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383428
Sequences flagged as poor quality0
Sequence length125
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG155454.0542161761791No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC129593.3797740384113837No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA119783.1239241787245584No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG85222.2225815537728075No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA71161.855889502070793No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA67831.769041384562421No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA66681.7390487914288992No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC65071.6970591610419687No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA62451.6287282097290756No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA61051.5922154876534838No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT57591.5019769030952357No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC57471.498847241203042No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG54191.413303149483084No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC51821.351492327112261No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC50241.3102851121983787No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA50021.3045473987293574No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC48891.2750764159112011No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT48381.2617753528693785No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA47921.2497783156159696No Hit
ACAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA45271.180664948830028No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC39091.0194873613820588No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC38611.0069687138132843No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG36570.9537644616459935No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG36570.9537644616459935No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG34750.9062979229477242No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG33780.8809998226524929No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTTGTCATATTTT32810.8557017223572614No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC32150.8384885819501966No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG29580.7714616564257174No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA29070.7581605933838947No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA28360.739643427188416No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA27430.7153885475239158No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA25720.6707908655601573No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT25520.6655747624065014No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG24520.6394942466382215No Hit
CCTCCTGGCCCCTGTCGAACCATGTACTTCAATGCCTGGCCGTATTGTAG24230.6319308970654204No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT23450.611588094766162No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT22430.5849859686825167No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC22320.5821171119480059Illumina Single End PCR Primer 1 (100% over 21bp)
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT22200.5789874500558123No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT22000.5737713469021564No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG21680.5654255818563068No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC19220.5012675130663384No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA18420.4804031004517146No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG17220.4491064815297787No Hit
TCCAGATGATTCTTTAACAGGTAGCCGGTAGCAGAGACTGGGTCGTAGCC17050.44467279384917113No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT16090.4196354987116225No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC16010.41754905745016013No Hit
GTTTCCTCTGGTCCTGGTATGAAGGCAATCTGAGTTATCTGTTGCTGTAG15860.4136369800849181No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC12540.3270496677342291No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCCTCCTGAGACTATCAAGAAAA12280.3202687336344764No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACTAAGGCTCA12180.31766068205764836No Hit
TTCTCTTCCGCACCCAGCCATCTGTAGAATGAGCCAGGCCTGCTAAATGT12140.3166174614269172No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG11550.3012299571236321No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT11550.3012299571236321No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT11140.29053694565863736No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT10480.2733238052515726No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC10140.2644564298903575No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG8900.2321165903376905No Hit
CCTGGTCCTCTGACTGCTCTGCTACCTACCAGAATGCCAGAGGCTGTGTA8860.23107336970695933No Hit
CCACACAGCAAAGCAGAAACTCACATTGAGGATTTCCTTGTTGGCTTTCG8660.22585726655330335No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT8490.22142357887269581No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA8250.2151642550883086No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC8010.20890493130392146No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCTACACCCC7980.20812251583087307No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7830.2042104384656311No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA7710.2010807765734375No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGTGCCTCTG7310.19064857026612558No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7150.1864756877432008No Hit
CTTGGGCCTGTGTTATCTCCTACGGTAGCAAAGACTTGGTCTCTTGGGCC7100.18517166195478682No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA6870.17917314332808246No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG6860.17891233817039967No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6810.17760831238198566No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA6810.17760831238198566No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC6650.17343542985906088No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6550.1708273782822329No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT6550.1708273782822329No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG6140.1601343668172382No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTGCTTGCCTCTGACCCCTGTAGAAC5570.14526847282931868No Hit
AACAGAGAATCTCCATTTTAGCACCATGTCGTCAGTGTATGTCGTCAGTG5440.1418780057794423No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5360.13979156451797992No Hit
CTTGGGCCTGTGTTATCTCCCTTGGTCTGCTTGCCTCTGACCCCTGTAGA5240.1366619026257863No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC5000.13040257884139916No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACCATGTCGTCAGTGTACTTCAATGC4890.12753372210688838No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC4820.12570808600310882No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT4770.1244040602146948No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC4710.12283922926859801No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT4610.12023117769177002No Hit
CCTCCTGGCCCCTGTCGTTCAGTGTACTTCAATGCCTGGCCGTATTGTAG4580.11944876221872164No Hit
CCTCCTGGCCCCTGTCGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC4530.11814473643030764No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT4500.11736232095725925No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA4460.11631910032652806No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTTTATACTGCAAATGCTATC4440.11579749001116245No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGGAAACCTACCAGGGC4210.1097989713844581No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAACTGCCCAACAACACCAGT4120.10745172496531293No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG4090.10666930949226451No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA4080.10640850433458171No Hit
CCAAGTCAACAACCCCCACAACACTGACTTCCATTTTCTACTTTTTCTGA4060.10588689401921612No Hit
CTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC4010.10458286823080212No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCTCCTG3860.10067079086556016No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3860.10067079086556016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)