FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379241
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT172974.560952006771419No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA129893.4249988793405772No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT101902.6869457679944944No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA93712.4709881051890488No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG92562.4406643796424965No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC88722.339409504774009No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC78672.07440651195414No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT75451.989500080423794No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG75401.988181657573944No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA67481.7793434781576887No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC59431.5670773993318232No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC58921.5536294862633522No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA57561.51776838474743No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA57331.5117036396381192No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT51291.3524381593762278No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT49601.3078754670512944No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC47621.2556659221972308No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA44151.1641673764176341No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA44141.1639036918476642No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG41051.0824251597269283No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC40461.066867770098697No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA38241.0083297955653527No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC37870.9985734664764622No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT37460.9877623991076914No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC34970.9221049411851567No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT33740.8896717390788443No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT33730.8894080545088744No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG33290.8778059334301934No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA31270.8245416502962497No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA29860.7873621259304769No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA29840.7868347567905368No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG29460.7768147431316761No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTGTGCTAAAATGGAG29380.7747052665719161No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT28970.7638941992031453No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG28760.7583568232337748No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG28210.7438541718854238No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTATCTCG27410.7227594062878223No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT26740.7050925400998309No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG26050.6868983047718996No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTAAGAGGAGGCGCAACTGCTTGC23090.608847672060774No Hit
GCAACAGATAACTCAGATTGCCTTCATACCAGGACCAGAGGAAACAGACC22940.6048924035112238No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT22440.5917081750127228No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGGCATTTTGAGGCATAACTGC20130.5307970393496484No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC19780.5215680794006977No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA18670.4922990921340256No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG18640.4915080384241155No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTAACACAACAATAATGA18360.484124870464955No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT16980.4477363998090924No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC16870.44483586953942217No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC16500.4350795404505315No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG16020.42242268109197056No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT15710.41424845942289995No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA14880.3923626401153884No Hit
GGAATCCTATGGCTTTCCAACCATACACTGACGACATGGTTCTACAGCAA14380.3791784116168874No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG13530.35676522316943576No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA13480.3554468003195857No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG13440.35439206203970564No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG13150.3467452095105751No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG12870.33936204155141453No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA12650.3335609810120741No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT12390.3267051821928536No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATAGCTT12000.31642148396402286No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT11370.2998093560559117No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA10590.2792419595982502No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT10270.2708040533592096No Hit
GGATGCAGAGCTTCTTCCCATAATGATCTGTCCCTCACAGCAGGGTCTTC9940.262102462550199No Hit
CAGAGGGGAAAAATATGACAAACACTGTGCTAAAATGGAGATTCTCTGTT9910.2613114088402889No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA9740.25682877115079855No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGGAACTTTCCACTTGATAAGAG9730.25656508658082855No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG8730.23019662958382667No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG8440.22254977705469609No Hit
ATACGGCCAGGCATTGAAGTACATGACGACAGGGGCCAGGAGGAGACCAA8290.2185945085051458No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC7750.2043555417267648No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAG7710.20330080344688467No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC7200.1898528903784137No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG7150.18853446752856365No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATAGCTTCA7140.18827078295859362No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT7000.18457919897901334No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG6760.17825076929973288No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT6540.17244970876039248No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATAGCTTCACTATCT6080.1603202185417716No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC5300.1397528220841101No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG4770.1257775398756991No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA4570.12050384847629872No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG4320.11391173422704824No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATAGC4310.11364804965707821No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATAGCTTC4300.1133843650871082No Hit
TGTCCTGCTTGCTTACCTCGCTTGGTGCTCCCTGGGGGCAGCTCGTGGTG4210.11101120395737804No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCTTCAGGGCAACTG3990.10521014341803761No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT3960.10441908970812755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)