FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665706
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACACTGCTTGAAT280444.212670458130165No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA202433.040831838679537No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT178262.67775865021496No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG166332.4985504111424564No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA157992.3732698818998177No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT156282.347582866911219No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC150302.257753422682085No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC133172.0004326234103345No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA128591.9316334838502283No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT119771.7991425644353514No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG115261.7313949401086968No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG113171.699999699567076No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA109061.6382607337172868No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG104751.5735174386290647No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG103271.5512854022646634No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC102681.5424226310112872No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT101701.5277014177429675No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA100071.5032161344497421No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC94181.4147386383779026No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT93281.401219156804956No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC91491.3743304101209843No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG90421.3582572486953701No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA88921.3357247794071256No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA86771.3034282400939754No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT85121.2786425238769066No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT81121.218555939108255No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT79941.2008303966015028No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG78391.1775468450036501No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT77771.1682334243645092No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT73261.1004858000378546No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG69941.0506139346798737No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA64240.964990551384545No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC63630.9558273472073258No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG58780.8829723631753358No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG55950.8404611044515146No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG52530.7890870744743175No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA51250.7698593673483489No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC50440.757691833932697No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT48010.7211892336857412No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC47340.711124730736992No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC45080.6771758103427039No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC42080.632110871766215No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACACTGCTTGAATCTCG40110.6025182287676542No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT39720.5966597867527106No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA37640.5654147626730118No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA33940.509834671762009No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC31390.47152947397199363No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA26600.3995757887115333No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG24640.370133362174894No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC21990.33032599976566235No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACACTGCTTGAAT18950.28466019534148707No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA14850.22307144595361916No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA13250.19903681204615853No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACACTGCTTGAATCT13250.19903681204615853No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG13000.1952814004981178No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACACTGCTT12780.19197663833584194No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACACT11980.17995932138211163No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA11770.17680477568175743No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACAC9320.1400017425109583No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC9080.13639654742483917No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACACT8570.1287355078668361No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG8220.1234779316995791No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG8060.12107446830883302No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA7850.11791992260847882No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG7650.11491559337004624No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTACACTGCTTGAAT7380.11085974889816226No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC7360.110559315974319No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7110.10680390442627827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)