FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329539
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGGACAGATGGAT149774.544833843642179No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA118723.602608492469783No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC117843.5759045211644143No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA114423.47212317813673No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA113643.4484537490251537No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC108483.2918713718254895No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG91562.7784268326358945No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA69372.105061919833464No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC66652.0225223721623236No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC63821.936644828078012No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG60701.8419671116317038No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG60101.823759858468952No Hit
TCCCTGCCAGCGAGATAGGTGGGTGGATCACCTGAGGCCTTTGCATCCCT58471.7742968207101435No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA56901.7266545082676104No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC56511.7148197937118217No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC51881.5743204901392551No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA51531.5636995924609833No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA50351.5278919945742386No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC49961.5160572800184502No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT47451.4398902709542725No Hit
CAGAGGGGAAAAATATGACAAGTCTCTGCTAGTGCTAAAATGGAGATTCT46351.406510306822561No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT36061.0942559150813713No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT32310.9804605828141737No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG31950.9695362309165227No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG30350.9209835558158519No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC25150.7631873617386713No Hit
GGAATCCTATGGCTTTCCAACCAGCAACAGAGGAGGGGGAGACCAAGTCT24070.7304143060457184No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT23620.7167588661736547No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT22790.6915721659651817No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG21540.6536403885427825No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA19230.5835424638661889No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGGACAGATGGATCTCG19110.5799010132336385No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA19000.5765630168204674No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT17970.5453072322244105No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG16540.5019132788531858No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA15630.4742989448896793No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGGACAGATGGATCT15110.4585193254819612No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC14850.4506295157781021No Hit
CAGAGGGGAAAAATATGACAAATCTCTGCTAGTGCTAAAATGGAGATTCT14780.44850533624244776No Hit
GGAAACCGTAGCTGCCCTGTTCATCTGGACCTGGGTCTTAGACCAAGTCT13280.40298720333556876No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT12750.38690412970847154No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGGACAGATGGAT12600.3823523164177836No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGGA12450.37780050312709573No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA11530.3498827149442099No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGGA10670.3237856520775993No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTAGGACAGAT9460.2870676915327169No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGGAC9310.28251587824202906No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGGAC8840.26825352993120694No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG8730.2649155335180358No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA8690.263701716640519No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA8600.26097062866610626No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA8340.25308081896224727No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGGACAGAT8190.24852900567155933No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGGA7970.2418530128452171No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG6860.20816959449412664No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGG6840.20756268605536826No Hit
GGAATCCTATGGCTTTCCAACCTTCTACAGCAACAGAGGAGGGGGAGACC6360.19299688352516697No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGGACAGATGGA6130.18601743647944552No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGATGGTTCTACAGCAACAGAG5750.1744861761430362No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGGACAGATGGAT5730.1738792677042778No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG5690.17266545082676102No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGGACAGATGGATCT5490.16659636643917716No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC5360.16265146158724764No Hit
GCAACAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTA5130.1556720145415262No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGGACAGAT4780.14505111686325442No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG4740.14383729998573763No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAGGACAGATGGAT4140.12563004682298606No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGGA4040.12259550462919411No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC4010.12168514197105652No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4000.12138168775167733No Hit
GGAAACCGTAGCTGCCCTGTTAAAAAATCATCTGGACCTGGGTCTTAGAC3870.11743678289974782No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGGA3860.11713332868036863No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC3810.11561605758347267No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGGACAGATGGAT3620.10985042741526799No Hit
CAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGGAC3320.10074680083389219No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGG3310.100443346614513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)