FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329539
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG151574.599455603130434No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC148594.509026245755434No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA112943.4272119536686096No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA112063.4005079823632407No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA107723.2688088511526705No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC90522.746867593820458No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA87682.6606865955167676No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA60261.8286151259790193No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG60191.8264909464433647No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA58081.762462106154355No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA58051.7615517434962173No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC57841.7551792048892543No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG55041.6702120234630802No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA52971.6073970000515871No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT51521.5633961382416042No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG48061.4584009783364034No Hit
AGGGATGCAAAGGCCTCAGGTGATCCACCCACCTATCTCGCTGGCAGGGA46731.4180415671589706No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC46721.4177381129395914No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTGTCATATTTTTCCC44011.33550201948783No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG43901.3321640230746588No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT34341.04206178934815No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG31660.9607360585545263No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA31470.9549704283863215No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT28790.8736446975926977No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT24940.7568148231317082No Hit
CCCCCTCCTCTGTTGCTGGTTGGAAAGCCATAGGATTCCTGTAGAACCAT24090.7310212144844768No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT22440.6809512682869099No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT22320.6773098176543596No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC19580.5941633615444606Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC19570.5938599073250814No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA17640.5352932429848971No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA17110.5192101693577998No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC16740.5079823632407697No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC16550.502216733072565No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG14940.45336060375251486No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG14290.4336360794928673No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGATTTGTCATATTTTTCCC14100.4278704493246626No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC13660.41451846367197814No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG12640.38356613329530037No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA12310.373552144055787No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT12090.3668761512294448No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT11730.35595179933179383No Hit
AAGACCCAGGTCCAGATGAACAGGGCAGCTACGGTTTCCTGTAGAACCAT11680.35443452823489785No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC8970.27219843478313643No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8710.26430862507927744No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC8640.2621844455436231No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG8530.2588464491304519No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT8360.25368772740100565No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8330.25277736474286805No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA7850.23821156221266676No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG7800.2366942911157708No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT7790.23639083689639162No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG7140.21666631263674405No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA6890.2090799571522642No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC6480.1966383341577173No Hit
CCCCCTCCTCTGTTGCTGTAGAAGGTTGGAAAGCCATAGGATTCCTGTAG6420.19481760884144214No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG6330.1920865208670294No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA6180.1875347075763415No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA6040.18328634850503278No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG6040.18328634850503278No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATCGTCAGTGGGTTGGAAAGCCATA5710.1732723592655194No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC5480.16629291221979797No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5310.1611341904903517No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC4950.1502098385927007No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCCTGCCGTC4880.14808565905704638No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTC4770.14474766264387523No Hit
GCCTCAAAGACAATGGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG4750.14414075420511685No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4240.128664589016778No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC4150.12593350104236525No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA3780.11470569492533508No Hit
CCAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG3760.1140987864865767No Hit
CCTGGTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3600.10924351897650961No Hit
AAGACCCAGGTCCAGATGATTTTTTAACAGGGCAGCTACGGTTTCCTGTA3390.10287098036954655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)