FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208666
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCCTTGTTCTAT82733.9647091524254074No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA79503.8099163256112636No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT60482.898411816012192No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC57902.7747692484640525No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA46242.2159815207077336No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG41491.9883450106869351No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC38271.8340314186307303No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG35451.6988872168920668No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC34631.6595899667411078No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA34011.6298774117489194No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG29561.4166179444662763No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG28721.3763622247994403No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT27551.320291758120633No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT26941.291058437886383No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA26481.2690136390212108No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC25501.2220486327432356No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT25361.2153393461320963No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA22521.0792366748775555No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA21741.0418563637583507No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT21671.0385017204527809No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC20921.0025591136073917No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT20720.9929744184486212No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC20650.9896197751430515No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA19300.9249230828213508No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG19040.9124629791149492No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA18510.8870635369442074No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA17490.838181591634478No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA17270.8276384269598305No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT15940.7639002041540068No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC15210.7289160668244946No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA14810.7097466765069537No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG13800.6613439659551629No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG13480.64600845370113No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC13360.6402576366058678No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG13100.6277975328994662No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG12620.604794264518417No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT12510.5995226821810932No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC12470.5976057431493391No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG12420.5952095693596465No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG11960.5731647704944745No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCCTTGTTCTATCTCG11750.5631008405777654No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT11520.5520784411451793No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA10940.524282825184745No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG10610.5084680781727737No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT10190.48834021833935576No Hit
TCAAATCATCCATTGCTTGGGTAGCAGAAAGTCAGTCCCATGGAGACCAA9740.4667746542321221No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT9650.4624615414106754No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG9400.45048067246221235No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC8790.4212473522279624No Hit
AGAAAACGGCATTTTGAGTGTGTACTGAGGCATAACTGCACCCTTAGACC7690.3685315288547248No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA7410.3551129556324461No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG6910.3311512177355199No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATCCTTGTTCTATCT6870.3292342787037658No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG6790.32540040064025766No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT6700.3210872878188109No Hit
GACCCAAAACCCAAAATGGCAGAGGAGGGAGAGATGGGGGTGGGAGGCTG6310.3023971322592085No Hit
TGACAGTTTGACAGTTAAAGGCATACACTGACGACATGGTTCTACATCAG6270.3004801932274544No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA6230.2985632541957003No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCGCTAATGAGTTTGTACTGA6220.2980840194377618No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC6050.2899370285528069No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGATGATAAAAG6020.2884993242789913No Hit
AGCTGCTCACCATCGCTACACTGACGACATGGTTCTACATTTCGCTTCCC5930.2841862114575446No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC5850.2803523333940364No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCCTTGTTCTAT5690.27268457726702No Hit
GCAACAGATAACTCAGATTGCCTTAATGTTAACTCATACCAGGACCAGAG5640.2702884034773274No Hit
ATACGGCCAGGCATTGAAGTCTGACGACAGGGGCCAGGAGGAGACCAAGT5630.26980916871938887No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA5600.2683714644455733No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACTCA5580.2674129949296963No Hit
GCAACAGATAACTCAGATTGCCTTATAACTCATACCAGGACCAGAGGAAA5300.2539944217074176No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA5150.24680590033833977No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG5040.241534318001016No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATCC4990.23913814421132337No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT4850.23242885760018403No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC4520.21661411058821275No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4510.21613487583027421No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCCT4500.21565564107233567No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG4050.19409007696510214No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCCT4000.19169390317540952No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG4000.19169390317540952No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCCTTGTT3930.18833925986983985No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA3820.18306767753251607No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATCCTTG3800.18210920801663902No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCC3680.17635839092137673No Hit
TTTCGCTTCCCACAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCG3600.17252451285786854No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT3450.16533599148879072No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT3430.16437752197291364No Hit
GAGAAAGCCCCCCTACTGCTACACTGACGACATGGTTCTACATCCCTTCC3370.1615021134252825No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA3310.15862670487765135No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG3270.15670976584589727No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT3170.15191741826651203No Hit
TCCACAAACAGAACAAGATGCTGTACCGTAATCCTGTTTGAAGGAAAAGG3120.14952124447681941No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTGA3080.1476043054450653No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTA3050.14616660117124974No Hit
AGCTGCTCACCATCGCTAACACTGACGAATGTTCTACAGGTCTTGGCCAG3020.14472889689743418No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATCC3020.14472889689743418No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC2940.140895018833926No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACAACGTGGTGGAT2920.13993654931804894No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA2710.12987261940133993No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATCCTTGTTCTAT2700.12939338464340142No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG2690.1289141498854629No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2650.1269972108537088No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT2640.12651797609577028No Hit
TCAAATCATCCATTGCTTGGTAACGGTAGCAGAAAGTCAGTCCCATGGAG2590.12412180230607765No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA2540.12172562851638503No Hit
GGGGTCAGAGGCAAGCAGAGCAAGGAGATAACACAGGCCCAAGAGACCAA2500.11980868948463094No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGACGACAGGGGCCAGGA2410.11549557666318422No Hit
AGATGAGTCATATTTGTGGGTTTTCATAGACCAAGTCTCTGCTACCGTAT2330.11166169859967603No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGA2320.11118246384173751No Hit
AGCTGCTCACCATCGCTACGTAGCAGGTCTTGGCCAGTTGGAGACCAAGT2200.10543164674647523No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG2140.10255623819884409No Hit
TCAGCCGTTACCTGTGTGTGAGACCAAGTCTCTGCTACCGTATCCTTGTT2130.10207700344090555No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATACCCTCCATGA2120.10159776868296704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)