FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208666
Sequences flagged as poor quality0
Sequence length125
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG83383.995859411691411No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC77343.7064016178965433No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA46342.220773868287119No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA42852.0535209377665744No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG39151.8762040773293205No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA37761.8095904459758658No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC35951.7228489547889931No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA33961.6274812379592267No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG31371.503359435653149No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG24191.159268879453289No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC23761.1386617848619325No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA22981.1012814737427277No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT22781.091696778583957No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC22711.0883421352783873No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT22481.0773197358458015No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC22061.0571918760123835No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT21611.0356263119051499No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG21401.025562381988441No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA21341.0226869734408097No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC21171.0145399825558548No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT20390.9771596714366501No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA20390.9771596714366501No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC20110.9637410982143713No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT19770.9474471164444614No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG18290.87652037226956No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG17090.8190122013169371No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT16390.7854657682612405No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT15890.7615040303643142No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA15690.7519193352055438No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA15590.7471269876261586No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT14410.6905772861894127No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA14060.6738040696615645No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT13400.6421745756376218No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA13260.6354652890264825No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG12530.6004811516969703No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA12090.5793948223476753No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG11930.5717270662206588No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT11870.5688516576730277No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC11780.564538544851581Illumina Single End PCR Primer 1 (100% over 21bp)
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT11750.5631008405777654No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT11680.5597461972721958No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA11390.5458483892919785No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT11300.5415352764705318No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC11070.5305128770379458No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC10960.5252412947006221No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT10790.5170943038156671No Hit
CCAACTGGCCAAGACCTGTGGGAAGCGAAATGTAGAACCATGTCGTCAGT9810.47012929753769184No Hit
CCATGGGACTGACTTTCTGCTACCCAAGCAATGGATGATTTGATGTAGAA9690.4643784804424295No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC9430.4519183767360279No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA9280.44472985536695003No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC7690.3685315288547248No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC6910.3311512177355199No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT6870.3292342787037658No Hit
AAGGGTGCAGTTATGCCTCAGTACACACTCAAAATGCCGTTTTCTTGTAG6530.312940296933856No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG6470.31006488838622487No Hit
CCTCCTGGCCCCTGTCGTCAGACTTCAATGCCTGGCCGTATTGTAGAACC6360.3047933060489011No Hit
CACACACAGGTAACGGCTGATGTAGAACCATGTCGTCAGTGTATGCCTTT6230.2985632541957003No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT6050.2899370285528069No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC5990.2870616200051757No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC5990.2870616200051757No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5700.27316381202495854No Hit
GGCCTCAGTACAAACTCATTAGCGACTTGGTCTGGACCTAATTACGAGTG5630.26980916871938887No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG5520.2645375863820651No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT5520.2645375863820651No Hit
GTTTCCTCTGGTCCTGGTATGAGTTAACATTAAGGCAATCTGAGTTATCT5470.2621414125923725No Hit
GTTTCCTCTGGTCCTGGTATGAGTTATAAGGCAATCTGAGTTATCTGTTG5190.24872283937009385No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT5170.2477643698542168No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT4680.2242818667152291No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT4610.22092722340965945No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG4550.2180518148620283No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC4470.2142179367985201No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA4280.20511247639768818No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA4110.19696548551273327No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC4080.19552778123891768No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT4050.19409007696510214No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC3930.18833925986983985No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA3720.17827532995313083No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG3640.17444145188962265No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT3600.17252451285786854No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT3310.15862670487765135No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT3230.1547928268141432No Hit
CCAACTGGCCAAGACCTGTAGAACATTCGTCAGTGTTAGCGATGGTGAGC3200.1533551225403276No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT3170.15191741826651203No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT3090.14808354020300385No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA3020.14472889689743418No Hit
AGCATCTTATCCGAGTGGAAGGGATGTAGAACCATGTCGTCAGTGTAGCA3010.14424966213949564No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA3010.14424966213949564No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATATCCCATTTCT2900.1389780798021719No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTACAGCATCTTGTTCTGTTTGTG2870.13754037552835632No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCAGCATCTTGTTCTGTTTG2860.1370611407704178No Hit
AGGGATGCAAAGGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTGTA2770.1327480279489711No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA2770.1327480279489711No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT2710.12987261940133993No Hit
CCATGGGACTGACTTTCTGCTACCGTTACCAAGCAATGGATGATTTGATG2640.12651797609577028No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2590.12412180230607765No Hit
CCTCCTGGCCCCTGTCGTCATGTCGTCAGTGTACTTCAATGCCTGGCCGT2570.12316333279020061No Hit
CTTGGGCCTGTGTTATCTCCTTGCTCTGCTTGCCTCTGACCCCTGTAGAA2410.11549557666318422No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC2360.11309940287349161No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG2220.10639011626235229No Hit
CCAACTGGCCAAGACCTGCTACGTAGCGATGGTGAGCAGCTTGTAGAACC2220.10639011626235229No Hit
CACACACAGGTAACGGCTGATGTAGAACCATGTCGTCAGTGTAGATCTCG2180.1044731772305982No Hit
ATGAGGTACTCGTCGGCATCTACCACGTCGTCCATGTCTTCTTCATCCAT2130.10207700344090555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)