FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520736
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG187903.6083543292570512No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC146042.804492103484299No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA127442.447305352424261No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC114172.192473729490567No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA98181.8854083451115344No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA96841.8596755361641983No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG93021.7863178270755238No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG84161.6161740306028392No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG80031.5368632089964973No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC68691.3190945123824742No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC68051.306804215571806No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC67871.3033475695938057No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA64171.2322942911571315No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT62811.206177410434462No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA62261.1956154366127942No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT61351.1781401708351256No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT57541.1049744976341178No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA57321.1007497081054507No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT53271.0229751736004424No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG52541.0089565538007743No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT52371.0056919437104406No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA51120.9816874577521046No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG47430.9108262152030971No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT46340.8898943034474283No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC42930.824410065753088No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT41890.8044383334357524No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA40330.7744807349597492No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG40190.7717922325324157No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG39270.7541249308670804No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA38980.7485558901247465No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC36540.7016991335340749No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT36310.6972823081177411No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT36230.6957460210164076No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA35150.6750061451484053No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT34880.6698211761814048No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC32080.6160511276347324No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG31050.5962714312050637No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA31040.596079395317397No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT30970.5947351441037302No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC30810.5916625699010631No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA30560.5868616727093959No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT30070.5774519142137282No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG27970.5371243778037239No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT26110.5014057026977201No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC25100.48201007804338475No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA24870.4775932526270509No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC24390.46837553001904997Illumina Single End PCR Primer 1 (100% over 21bp)
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT22730.4364975726663799No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT21570.4142214096970442No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC20170.38733638542370796No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT19620.3767744116020402No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG19580.37600626805137344No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT18510.3554584280710379No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG18270.35084956676703744No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA18210.3496973514410373No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA17040.3272291525840349No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC16290.3128264610090334No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT16170.31052203035703313No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG14640.28114053954402995No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG13830.2655856326430283No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC13600.2611688072266945No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT12340.23697228538069195No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC11490.22064923492902352No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT11220.215464265962023No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGTGGAGCCGCAGTCAGATC11200.2150801941866896No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC11160.21431205063602285No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT10650.20451822036502182No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT10380.19933325139802124No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT10070.19338013888035396No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG9280.17820930375468566No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG9120.1751367295520187No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG8930.17148804768635165No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC8500.16323050451668408No Hit
GGCCTTTTCCTTCAAACAGGATTCGGTAGCAGAGACTTGGTCTAGCATCT8490.16303846862901739No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8470.16265439685368402No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT8380.16092607386468383No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG8070.15497296134701652No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8000.15362871013334972No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA7670.14729152584034905No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC7610.14613931051434892No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT7600.14594727462668222No Hit
CCATGGGACTGACTTTCTGCTACCCGTACCAAGCAATGGATGATTTGATG7540.14479505930068212No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTTC7530.14460302341301542No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT7510.14421895163768203No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7410.14229859276101517No Hit
CCTCCTGGCCCCTGTCCGTATTGTAGAACCATGTCGTCAGTGTACTTCAA7190.13807380323234805No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA7150.13730565968168132No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGCGATGGTGAG7150.13730565968168132No Hit
CTTGGGCCTGTGTTATCTCCCTTGGTCTGGTCTTTGCCTGCTTGCCTCTG6410.12309500399434647No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6150.11810207091501261No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA6100.11714189147667917No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT5930.11387728138634548No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGATAATCTACTGGGACG5610.1077321329810115No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT5520.10600380999201131No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGATAGAGATTTCA5460.1048515946660112No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5340.10254716401401093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)