FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463341
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG175093.7788583354376155No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC130152.8089463267873986No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA111162.3990969933591026No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA85841.8526312154547082No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG81321.7550788727956301No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC77261.6674544234160154No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA76801.6575265301365518No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG76021.6406922763148524No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGG75571.6309802068023336No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG71311.5390392820838217No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC66951.4449401196958611No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG64551.393142415629094No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA62081.3398339451937127No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC61891.3357332936217603No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT60671.3094027940544868No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC58981.272928577440805No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC54721.1809876527222931No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA53381.1520672679516815No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT53181.1477507926127841No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT52481.1326431289266436No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT52261.1278950060538566No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG50131.0819245436946008No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT50031.0797663060251521No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT45900.9906310902769234No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT44850.9679695947477128No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA44580.9621423530402015No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT42280.9125028866428828No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA42230.9114237678081586No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT42210.9109921202742689No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC40440.872791313525028No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC39230.8466766377246995No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG37990.8199144906235365No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG37930.8186195480218672No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT36940.7972529950943257No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA36020.7773972085353983No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT32990.7120026071511046No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT29850.6442339443304176No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT28440.6138027931911918No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT28070.6058173138142319No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA27910.602364133543114No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT27830.6006375434075552No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA27800.5999900721067206No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG27150.5859615272553044No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC26510.5721488061708332No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT26380.5693430972005499No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC26030.5617892653574796No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT25720.5550987285821889No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA24740.5339479994215923No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC23390.5048117908840357Illumina Single End PCR Primer 1 (100% over 21bp)
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG19490.420640521775539No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC17810.3843821289288019No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC16920.36517381367070906No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC16630.358914924429308No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA16260.3509294450523481No Hit
CAGCCAGACTGCCTTCCGAGACCTGTGGGAAGCGAAATGTAGAACCATGT14210.30668557282865105No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG12170.2626575243718989No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC9040.1951046853181566No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC9020.1946730377842669No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG8750.18884579607675556No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC8500.18345020190313396No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT8010.17287483732283565No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA7450.1607887063739233No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA7370.15906211623836441No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA7190.15517728843335685No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT7080.15280322699696336No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT7060.15237157946307364No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT6940.14978169425973528No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG6550.14136456734888558No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6340.13683226824304345No Hit
GTTCAAATGAGTAGACACAGCTTATCCATTTAGCAAGTGTGCTCCCGAAT6320.13640062070915374No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT6120.13208414537025645No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT5630.12150878078995815No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA5580.12042966195523383No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC5310.11460242024772252No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT5210.11244418257827388No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA4970.10726441217159717No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC4930.10640111710381771No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA4690.10122134669714097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)