FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340058
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGAGAGGACATAT192735.667562592263672No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT137224.0351939963182755No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC123163.621735115774368No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCAACAATAATGAGTCAT119493.513812349657999No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA113623.341194737368331No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC105273.0956483893923976No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTAGTGCTAAAATGGAGATTC68502.0143622558504726No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA66281.949079274711961No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC64901.9084979621123457No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA64521.897323397773321No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC58341.7155896935228696No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA57201.6820660005057961No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG56841.6714795711319834No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA53461.5720847620111864No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA52641.5479712284375018No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG46931.3800586958695282No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA43601.2821342241617606No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC41351.2159690405754313No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA38351.1277487957936587No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG33450.9836557293167635No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGAGAGGACATATCTCG30600.8998464967740797No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG27920.8210364114356963No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG27130.7978050803098294No Hit
GGGGTCAGAGGCAAGCAGGACATGGGAGATAACACAGGCCCAAGAGACCA25430.7478136082668251No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT25010.7354627739973769No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT24050.7072322956672097No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG23450.6895882467108552No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG23270.6842950320239488No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA22500.6616518358632939No Hit
TCTGTATCAGGCAAAGTCATAGAACCATGGGAGCCATTGTCTTTGAGGCA22260.6545942162807521No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA20870.6137188361985308No Hit
GGAATCCTATGGCTTTCCAACCGTACAGCAACAGAGGAGGGGGAGACCAA19670.5784307382858218No Hit
TCCACAAACAGAACAAGATGCTGCTACCGAATCCTGTTTGAAGGAAAAGG19530.5743137935293391No Hit
TTTCGCTTCCCACAGGTCTCGGAAGGCAGTCTGGCTGAGACCAAGTCTCT19430.57137311870328No Hit
TGAGCAAGAGGCTTTGGAGTGGGTCACTCTCAGCAGGCAAAGACCAGACC19390.5701968487728564No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA19390.5701968487728564No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG19250.5660799040163738No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT19140.5628451617077087No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT15580.45815713790000534No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGGTGCAGATAATGACAAGG15520.4563927330043699No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT15440.4540401931435226No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAG14980.4405130889436508No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG14470.4255156473307494No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAG13480.3964029665527645No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGAGAGGACATATCT12710.3737597703921096No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGAGAGGACATAT12600.37052502808344456No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG12370.3637614759835087No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA12070.3549394515053314No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG11310.3325903228272824No Hit
GGATGCAGAGCTTCTTCCCACAGGTCTCTGGGTGCGGAAGAGAAAGACCA10470.3078886542883861No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATACCCTCCATGA10410.3061242493927507No Hit
AGCTGCTCACCATCGCTAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCT9590.28201071581906617No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC9170.269659881549618No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACATGGTTCGACAGGGGCCAGG9130.2684836116191944No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGAGAGGAC9040.2658370042757412No Hit
AGCTGCTCACCATCGCTACAGGTCTTGGCCAGTTGGAGACCAAGTCTCTG8700.2558387098671403No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG8630.25378023748889894No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAGAGGACATA8610.2531921025236872No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG8560.2517217651106576No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGAGA8390.24672261790635716No Hit
GGGGTCAGAGGCAAGCAGGAATTGGTGGAACTGGTTGGGGAGATAACACA8350.24554634797593353No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGAGA8170.24025313328902717No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA7650.22496162419351992No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC7470.2196684095066136No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT6940.20408283292850043No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT6900.2029065629980768No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA6860.2017302930676532No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA6750.19849555075898817No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGAG6680.1964370783807468No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGAGAGGACATAT6390.18790912138517546No Hit
GGGGTCAGAGGCAAGCAGAGGAGATAACACAGGCCCAAGAGACCAAGTCT5830.1714413423592446No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGAG5780.16997100494621506No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG5760.16938286998100324No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA5650.16614812767233825No Hit
CAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAGA5110.15026848361161918No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG5080.14938628116380148No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTAGAGAGGAC4740.1393879867552006No Hit
GAGAAAGCCCCCCTACTGCTCCGCACCTTCCACTCGGATAAGATGCTAGA4570.13438883955090014No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGAGAGGACATATCT4380.12880155738138788No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGA4190.12321427521187563No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC4170.12262614024666381No Hit
GGAATCCTATGGCTTTCCAACCTTCTACAGCAACAGAGGAGGGGGAGACC3910.11498038569891018No Hit
GAGAAAGCCCCCCTACTGCCCGCACCTTCCACTCGGATAAGATGCTAGAC3800.11174564339024519No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG3750.11027530597721566No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAGAGGACATAT3710.10909903604679203No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGAGAGGAC3660.10762869863376247No Hit
TCTTAAATGGCTACGACCCAGAACCCAAAATGGCTACCTGTTAAAGAATC3620.10645242870333885No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATACCCTTTTGA3420.10057107905122069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)