FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340058
Sequences flagged as poor quality0
Sequence length125
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG194475.718730334237101No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA122513.602620729404984No Hit
CTCGATAATCTGGATGACTCATTATTGTTGGCACAACTAAAAGAAAAGTT115053.383246387380976No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA113093.325609160790218No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC111863.2894388604296916No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA80712.373418652112287No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA74142.180216316040205No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTTGTCATATTTTTCC64601.8996759376341683No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA61161.798516723617736No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA57921.7032388592534216No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG57881.7020625893229977No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG55981.6461897676278752No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA55911.6441312952496339No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA46381.363884984326203No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC43711.2853689664704258No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA38741.139217427615289No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA37861.1133394891459691No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC34281.008063330373054No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG33150.9748337048385864No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA31390.9230778278999464No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC31050.9130795334913455Illumina Single End PCR Primer 1 (100% over 21bp)
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT29700.8733804233395479No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC26700.7851601785577753No Hit
CTTGGGCCTGTGTTATCTCCCATGTCCTGCTTGCCTCTGACCCCTGTAGA24740.7275229519670174No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG24580.7228178722453228No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT24380.7169365225932047No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG24190.7113492404236924No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC23270.6842950320239488No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG23250.683706897058737No Hit
GCCTCAAAGACAATGGCTCCCATGGTTCTATGACTTTGCCTGATACAGAT22360.6575348911068112No Hit
CCCCCTCCTCTGTTGCTGTACGGTTGGAAAGCCATAGGATTCCTGTAGAA19810.5825476830423046No Hit
CAGCCAGACTGCCTTCCGAGACCTGTGGGAAGCGAAATGTAGAACCATGT19530.5743137935293391No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA19470.5725493886337036No Hit
GGTCTTTGCCTGCTGAGAGTGACCCACTCCAAAGCCTCTTGCTCATGTAG19040.5599044868816496No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT18890.5554934746425609No Hit
GGCCTTTTCCTTCAAACAGGATTCGGTAGCAGCATCTTGTTCTGTTTGTG18650.5484358550600192No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC15490.4555105305565521No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT15280.449335113421828No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA14810.4355139417393504No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTTACCCAAAAGTGAAAC14330.4213987025742667No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC14260.4193402301960254No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA14200.4175758253003899No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG12490.3672902857747796No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC12210.3590563962618142No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT12000.3528809791270901No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT11820.34758776444018374No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT11730.34494115709673057No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA11730.34494115709673057No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC11420.3358250651359474No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA11340.33347252527510013No Hit
AGGGATGCAAAGGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTGTA10880.31994542107522833No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC9840.2893624028842139No Hit
CCAACTGGCCAAGACCTAGCGATGGTGAGCAGCTTGTAGAACCATGTCGT9460.27818783854518936No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGACTTCAATGCCTGGCCG9100.26760140917137665No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA9060.266425139240953No Hit
CTTGGGCCTGTGTTATCTCCCCAACCAGTTCCACCAATTCCTGCTTGCCT8590.25260396755847536No Hit
CCAACTGGCCAAGACCTGTAGCGATGGTGAGCAGCTTGTAGAACCATGTC8580.2523099000758694No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG8480.2493692252498103No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG7940.23348958118909127No Hit
TTCTCTTCCGCACCCAGAGACCTGTGGGAAGAAGCTCTGCATCCTGTAGA7840.23054890636303219No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT7520.22113874691964314No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT7350.21613959971534266No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC6860.2017302930676532No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT6820.2005540231372295No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT6760.19878961824159408No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT6720.19761334831117044No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6710.19731928082856454No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA6680.1964370783807468No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6220.18290997418087504No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA6180.18173370425045138No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG6000.17644048956354505No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC5840.1717354098418505No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC5690.16732439760276188No Hit
CTTGGGCCTGTGTTATCTCCTCTGCTTGCCTCTGACCCCTGTAGAACCAT5560.16350152032888507No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCACCCAC5290.15556169829852554No Hit
CCTGGTCCTCTGACTGCTCTGTTGTGTAGAACCATGTCGTCAGTGTAGAT5020.14762187626816603No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC4960.14585747137253058No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC4830.14203459409865377No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT4420.1299778273118115No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG4370.12850748989878197No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA4010.11792106052496928No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG3970.11674479059454564No Hit
AGCATCTTATCCGAGTGGAAGGTGCGGAGCAGTAGGGGGGCTTTCTCTGT3970.11674479059454564No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC3950.11615665562933382No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC3870.11380411576848656No Hit
CCCCCTCCTCTGTTGCTGTAGAAGGTTGGAAAGCCATAGGATTCCTGTAG3830.11262784583806291No Hit
CCTGGTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3700.1088049685641861No Hit
GCCTCAAAGACAATGGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG3630.10674649618594476No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAAGGCAATCTGAGT3600.10586429373812703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)