FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465147
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCCAGACATAT187474.030338796122516No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA143033.074941900087499No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT116042.4946952253803634No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA115282.4783563045660832No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC103192.218438472138915No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC93632.0129120471592854No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG76491.6444263856372285No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA72001.5478977613528626No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG69841.5014608285122768No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT68471.4720077738865347No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA63291.3606451293892037No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC58001.246917641089806No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA57481.2357383794800354No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG56851.222194274068198No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT54171.1645780796178413No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC53361.1471642298026214No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA52801.1351250249920992No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA52331.1250206923832682No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA50391.083313447146816No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC48691.0467658611148734No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC47831.0282770822987142No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG47711.0256972526964594No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT47611.0235473946945806No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA47561.0224724656936408No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT46430.9981790702724085No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT45870.9861398654618863No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT41830.8992856021859755No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA40270.8657478173566636No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG39490.8489789249420076No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT39150.8416694077356189No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG38940.8371547059316732No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG38390.8253304869213388No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA37140.7984572618978516No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG35880.7713690510741765No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG35860.7709390794738007No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC34810.7483655704540715No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG34300.7374012946444887No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT34270.736756337243925No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC34220.7356814082429856No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG31670.6808600291950717No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC30510.6559216763732756No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC27500.5912109505167183No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA27110.5828265043093903No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTCCAGACATATCTCG26130.5617578958909764No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC24640.5297250116629797No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT24620.5292950400626039No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG23310.5011319002379893No Hit
TCAAATCATCCATTGCTTGGCCGTATTGTAGAAGCAGAAAGTCAGTCCCA22750.48909269542746703No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG22580.4854379368242728No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT22120.47554859001562944No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG21670.46587422900717407No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG20660.4441606631881964No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC19330.4155675517632061No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG17800.3826747243344577No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG17330.3725703917256265No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA17270.3712804769244991No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT15700.33752770629499923No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCCAGACATAT12900.2773316822423879No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA12340.2652924774318656No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG11550.2483085992170217No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA11420.24551378381457906No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTCCAGACATATCT10940.23519446540555994No Hit
TGACAGTTTGACAGTTAAAGGCATTCCCTGTGAAATAATACTGGTATGTA10890.23411953640462047No Hit
TCACAATTGCCAGTTAACGTCTTACACTGACGACATGGTTTCTGCTTTGC10490.22552010439710457No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTC9790.21047109838395173No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG9720.20896619778263648No Hit
GACCCAAAACCCAAAATGGCAGGGAAGGGAGAGATGGGGGTGGGAGGCTG9710.20875121198244856No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT9610.2066013539805696No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTCCAGAC9610.2066013539805696No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT9480.20380653857812692No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA8860.19047741896647727No Hit
TCAAATCATCCATTGCTTGGTACGGGTAGCAGAAAGTCAGTCCCATGGAG8690.18682266036328302No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTCC8680.1866076745630951No Hit
ACTTCTACCGTGCCCTGACACACTGACGACATGGTTCTACAAGCTGTGTC8650.18596271716253143No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA8100.17413849815219704No Hit
TTTCGCTTCCCACAGGTCTCAGTGTCGGAAGGCAGTCTGGCTGAGACCAA8060.17327855495144545No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC7900.1698387821484391No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG7700.16553906614468114No Hit
TCAAATCATCCATTGCTTGGCCAGTGCATACCGGTAGCAGAAAGTCAGTC7590.16317422234261428No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA7470.1605943927403595No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTC7330.15758459153772894No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT7180.15435980453491047No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA7160.15392983293453466No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTCC7060.1517799749326557No Hit
CAGAGGGGAAAAATATGACAAAGTCGTGCTAAAATGGAGATTCTCTGTTA6940.14920014533040094No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC6840.14705028732852196No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG6740.144900429326643No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT6740.144900429326643No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT6580.1414606565236366No Hit
AGCTGCTCACCATCGCTACGGTAGGTCTTGGCCAGTTGGAGACCAAGTCT6470.13909581272156973No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGGGG6410.13780589792044237No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCTTCCAATTCAGGACCCA6360.13673096891950287No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGTCCAGACATAT6320.1358710257187513No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA6030.12963643751330226No Hit
CAGCCTCTGGCATTCTGGAGAGCAGTCAGAGGACCAGGAGACCAAGTCTC5690.12232692030691374No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTC5640.12125199130597424No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAGTTCACTGTA5510.11845717590353157No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG5200.11179261609770674No Hit
AGCTGCTCACCATCGCTAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCT5110.10985774389601567No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCGTGGACAAC5100.10964275809582777No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACGTGCTAAAATGGAGATT4770.10254822668962714No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG4740.10190326928906346No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCATACCAGGAC4710.10125831188849976No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTCCAGACATA4680.10061335448793608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)