FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428117
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATTGCACTCAGAT242625.6671423933177145No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA122312.8569292973649727No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT113162.643202675904017No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA98062.2904953552416747No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC95822.2381732096599762No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG87572.0454688788345243No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC84441.9723580236243832No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG66451.5521457919213673No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT63431.4816043277888988No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA59981.4010188803528008No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG56501.3197326898955193No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA55691.3008126283235657No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC55291.2914693880411197No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC54671.2769873656033282No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT50101.1702408453763806No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA48421.130999236190107No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC48111.1237582249712113No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC45941.0730711464389409No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA45831.0705017553612681No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC44021.028223593083199No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG43791.0228512299207926No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA43511.0163109617230803No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG43221.009537112518307No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT41690.9737992184379504No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT40690.950441117731835No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT39890.9317546371669427No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG37430.8742937094298988No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA37370.8728922233875318No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA36570.8542057428226396No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATTGCACTCAGATCTCG36190.8453296645543158No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA35390.8266431839894235No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG33670.7864672507749051No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG33340.7787590775418869No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG31510.7360137532496958No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT30610.7149914626141919No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG29940.6993415351410945No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC28840.6736476243643678No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT28480.665238708110166No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA27730.6477201325805796No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT27150.6341724341710326No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG27030.6313694620862988No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC24500.572273467299827No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG22970.5365355732194704No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT21630.5052357182732757No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG21480.5017320031673584No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA20910.48841788576487266No Hit
CAGAGGGGAAAAATATGACAAAACACTGACGACATGGTGCTAAAATGGAG20640.4821111985742216No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA19970.46646127110112423No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC19970.46646127110112423No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA19910.4650597850587573No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT19390.45291357269157734No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT18850.44030019831027495No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA17650.41227047746293655No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG16830.3931168348839219No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATTGCACTCAGAT14880.3475685385069969No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG14830.34640063347169114No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC14240.332619354055083No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG13460.31440003550431306No Hit
GGGGTCAGAGGCAAGCAGGGAAGACCAGGAGATAACACAGGCCCAAGAGA12380.28917328674170845No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT11970.2795964654522011No Hit
TTTCGCTTCCCACAGGTCTCTGCTACGGAAGGCAGTCTGGCTGAGACCAA11800.27562558833216155No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC11640.2718882922191831No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTGGGGGTTGTTGACTTGTAGACCAA11510.2688517391273881No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA11210.2618443089155535No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATTGCACTC11200.2616107279084923No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT10990.2567055267602081No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATTGC10140.23685114116001002No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATTG9490.22166837570103498No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATTGCACTCAGATCT9210.21512810750332267No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT9010.2104564873620996No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT8400.19620804593136923No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT8230.1922371688113296No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC7880.18406183356418923No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGCTGTGTCTACTCATTTGAACAG7690.1796237944300273No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG7520.1756529173099877No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATTGCACTCAGAT7510.17541933630292653No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA7510.17541933630292653No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATTGC7310.17074771616170348No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC6780.1583679227874623No Hit
GACCCAAAACCCAAAATGGCCGGGGAGGGAGAGATGGGGGTGGGAGGCTG6660.15556495070272847No Hit
AGAAAACGGCATTTTGAGTGTGTACTGAGGCATAACTGCACCCTTAGACC6530.15252839761093345No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA6400.14949184451913847No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG6360.14855752049089385No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA6220.1452873863920377No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG5660.13220684999661309No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA5660.13220684999661309No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATTG5580.13033820194012385No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG5530.12917029690481807No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA5210.12169570467886115No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC5160.1205277996435554No Hit
GCAACAGATAACTCAGATTGCCTTCATACCAGGACCAGAGGAAACAGACC5030.11749124655176038No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4770.11141814036817038No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCGTGGACAAC4760.11118455936110923No Hit
TCACAATTGCCAGTTAACGTCTAGACTTGGTTTCTGCTTTGCTGTGTGGA4700.1097830733187423No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATACTGAGG4690.10954949231168115No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA4640.10838158727637537No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATTGCAC4490.10487787217045809No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATTG4450.10394354814221346No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATTG4420.10324280512103001No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTATTGCACTCAGATCTCG4380.10230848109278538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)