FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180929
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA84084.6471267734857316No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA79344.385145554333468No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA78264.325453630982319No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT74414.112662978295353No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA60653.3521436585621984No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGACGTCTGCTAT56793.138800302881241No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC49872.7563298310386948No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT49382.729247384333081No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA43462.402047211889747No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC39722.1953362921366946No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA31991.7680968777807868No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG31001.7133792813755673No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT29371.6232886933548518No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG27401.5144062035384047No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT26981.4911926777907356No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC25281.3972331688120754No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC22771.2585047173200536No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT22311.2330803795964163No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG20931.156807366425504No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG19161.0589789364888988No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA17430.9633613185282626No Hit
GGAATCCTATGGCTTTCCAACCGTTCTACAGCAACAGAGGAGGGGGAGAC17360.9594923975703176No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA17350.9589396945763256No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC17140.9473329317024911No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG16070.8881937113453343No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG14410.7964450143426427No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA13260.7328841700335491No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG12160.672086840694416No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGACGTCTGCTATCTCG11950.6604800778205816No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG11880.6566111568626367No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA11780.6510841269227156No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGGCCA10730.5930503125535431No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGACGTCT10050.555466508962079No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACAGGGGCCAGGAGGAGACCAA9750.5388854191423156No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG9510.5256205472865046No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT9390.5189881113585992No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT8510.47035024788729285No Hit
CAGCCTCTGGCATTCTGGGAACTTCATCTGGACCTGGGTCTTCAGTGAAC8100.447689425133616No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC8010.44271509818768695No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG7950.43939888022373413No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGATCTTCAGTGAAC7590.41950157244001796No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC7370.4073421065721913No Hit
GGGGTCAGAGGCAAGCAGGAGGAGACCAAGTCTCTGCTACCGGAGATAAC7180.3968407496863411No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGAC7060.3902083137584356No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC6890.38081236286056963No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCCAGCAGTTCAACTTCTGTAACACC6470.35759883711290064No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG6170.3410177472931371No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGACGTCTGCTATCT6050.33438531136523164No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG6050.33438531136523164No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA5960.3294109844193026No Hit
GGGGTCAGAGGCAAGCAGGACATGGGAGATAACACAGGCCCAAGAGACCA5600.3095136766355863No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG5020.27745690298404346No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAAACATGGACGACGTGGTGGAT4960.27414068502009076No Hit
GAGAAAGCCCCCCTACTGCCTTCTACATCCCTTCCACTCGGATAAGATGC4880.2697190610681538No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCTTTTACT4790.2647447341222247No Hit
ATACGGCCAGGCATTGAAGTACGCTGACGACATGGTACACTGACGACAGG4030.22273930657882376No Hit
ATACGGCCAGGCATTGAAGTACACCGACGACAGGGGCCAGGAGGAGACCA3950.21831768262688678No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGTCTCTGGGAGATAACAC3940.21776497963289465No Hit
GGGGTCAGAGGCAAGCAGACCAAGGGAGATAACACAGGCCCAAGAGACCA3750.2072636227470444No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3660.20228929580111535No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGACGTCTGCTAT3650.20173659280712325No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGAC3600.19897307783716267No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC3290.18183928502340696No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC3070.16967981915558036No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGA2950.16304738322767492No Hit
TCCACAAACAGAACAAGATGCTGCTACCGCAATCCTGTTTGAAGGAAAAG2760.1525460263418247No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA2660.14701899640190352No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGACGTCTGCTA2540.1403865604739981No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA2490.1376230455040375No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGACGTCTGCTAC2410.13320142155210055No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA2360.13043790658213997No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGACGTCTGCTATCT2320.12822709460617146No Hit
GGGGTCAGAGGCAAGCAGGAGGAGACCAAGTCTCTGCTACCGTAGACGTC2210.12214736167225818No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGAC2190.12104195568427394No Hit
ATACGGCCCGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA2080.11496222275036064No Hit
GCAACAGAAGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA1850.10225005388854191No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA1820.10059194490656556No Hit
AGGACAAACTGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1820.10059194490656556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)