FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180929
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT79264.3807239303815315No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA78774.353641483675917No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC77334.274052252541051No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA77324.27349954954706No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG62893.475949129216433No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA44482.458422917276943No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG42042.3235633867428658No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT41212.2776890382415202No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC40682.248395779559938No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA37312.0621348705845937No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC33831.8697942286753366No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG30711.697350894549796No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA30611.6918238646098747No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT27721.5320926993461523No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA26981.4911926777907356No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT24971.3800993759983198No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC23761.3132223137252734No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG22261.2303168646264557No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA21751.2021290119328576No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT19811.0949046310983865No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC19561.0810870562485837No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA19431.0739019173266862No Hit
CCCCCTCCTCTGTTGCTGTAGAACGGTTGGAAAGCCATAGGATTCCTGTA17810.9843640322999629No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC16170.8937207412852555No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG15900.8787977604474683No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT15700.867743700567626No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG13510.7467017448833521No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC13300.7350949820095176Illumina Single End PCR Primer 1 (100% over 21bp)
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG11850.6549530478806603No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA11530.6372665520729126No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT10950.6052097784213697No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA10260.5670732718359136No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC9890.5466232610582051No Hit
CCTCCTGGCCCCTGTCGTCAGTGACTTCAATGCCTGGCCGTATTGTAGAA9740.5383327161483235No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC9500.5250678442925125No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG9450.522304329322552No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT8320.45984889100144255No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8290.45819078201946617No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA7800.4311083353138524No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG7510.41507994848808094No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA7120.3935245317223883No Hit
TCATTACACCAGTTCGTCCCTTCCCAGCTTTACAGTGAATTGCTGCAACA6920.38247047184254596No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA6710.37086370896871146No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC6170.3410177472931371No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC5990.331069093401279No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC5970.32996368741329474No Hit
CTTGGGCCTGTGTTATCTCCCATGTCCTGCTTGCCTCTGACCCCTGTAGA5490.30343394370167304No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT5430.30011772573772033No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT4430.2448474263385085No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTACAAATGCTATC4410.24374202035052422No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG4300.23766228741661094No Hit
AGCATCTTATCCGAGTGGAAGGGATGTAGAAGGCAGTAGGGGGGCTTTCT4290.2371095844226188No Hit
CTTGGGCCTGTGTTATCTCCCAGAGACTTGGTCTCTTGGGCCTGCTTGCC4180.2310298514887055No Hit
CCTCCTGGCCCCTGTCGTCGGTGTACTTCAATGCCTGGCCGTATTGTAGA4090.22605552454277644No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACCATGTCGTCAGCGTACTTCAATGC4060.2243974155608001No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3950.21831768262688678No Hit
CTTGGGCCTGTGTTATCTCCCTTGGTCTGCTTGCCTCTGACCCCTGTAGA3760.20781632574103653No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC3750.2072636227470444No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA3540.1956568598732099No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT3320.18349739400538334No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG2980.1647054922096513No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA2950.16304738322767492No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG2750.15199332334783255No Hit
GGCCTTTTCCTTCAAACAGGATTGCGGTAGCAGCATCTTGTTCTGTTTGT2710.1497825113718641No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG2570.14204466945597444No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC2560.14149196646198234No Hit
TCATTACACCAGTTCGTCCCTTCCAGCTTTACAGTGAATTGCTGCAACAT2530.13983385748000596No Hit
GCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC2520.13928115448601383No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2460.13596493652206115No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2380.1315433125701242No Hit
CCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCT2380.1315433125701242No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA2200.12159465867826605No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT2110.116620331732337No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG1990.10998789580443157No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCCATC1980.10943519281043945No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC1870.10335545987652614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)