FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192603
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA85104.418415081800387No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAT79574.13129598189021No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA73673.8249663816243777No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT57502.9854155958110726No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC56992.958936257483009No Hit
GGAATCCTATGGCTTTCCAACCGTACACTGACGACATGGTTCTACAGCAA53582.7818881325836045No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA45592.3670451654439444No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA40412.0980981604647906No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA36911.9163772111545512No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA36721.9065123596205669No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT36301.8847058457033379No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC32201.6718327336542007No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC31941.6583334631340114No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA30631.5903179078207503No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA30421.5794146508621363No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT30331.5747418264513016No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA28561.4828429463715518No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG26421.3717335659361485No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG25091.3026796051982574No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT23561.2232415902140672No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG22001.142245967092932No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT20591.0690383846565215No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC20321.0550199114240173No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA19521.0134836944388197No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG18790.9755818964398271No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA18460.958448206933433No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT16930.8790101919492428No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAATCTCG16910.8779717865246127No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14770.7668624060892094No Hit
AGAAAACGGCATTTTGAGTGTTAGACTCGAAACTTTCCACTTGATAAGAG13060.6780787422833497No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG12860.6676946880370502No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG11320.587737470340545No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG10790.5602197265878517No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTA10260.5327019828351583No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG9150.4750704817681968No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG8860.46001360311106265No Hit
GGAAACCGTAGCTGCCCTGTAGTACACTGACGACATGGTTCATCTGGACC8820.4579367922618028No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGAC8300.4309382512214244No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT8160.4236694132490148No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA7980.4143237644273453No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTA7620.39563246678400654No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTACTCGCGAATCT7510.38992123694854186No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC7030.3649995067574233No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAT6970.3618842904835335No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGTACTCG6920.35928827692195864No Hit
GGGGTCAGAGGCAAGCAGGGAAGAGGAGATAACACAGGCCCAAGAGACCA6470.33592415486778504No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC6190.3213864789229659No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG5870.3047719921288869No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCTACACTCTCAGC5800.30113757314268214No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAA5690.29542634330721745No Hit
ATACGGCCAGGCATTGAAGTCACAGAGTTGAACATTCCCTTTGAGAGAGC5290.2746582348146187No Hit
GGAATCCTATGGCTTTCCAACCGCACACTGACGACATGGTTCTACAGCAA4730.2455828829249804No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA4520.23467962596636605No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTA4170.21650753103534212No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGT4150.2154691256107122No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACATGGTTCGACAGGGGCCAGG4130.21443072018608225No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA3740.19418181440579846No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTAGGAAGGGACAGAAGATGAC3420.17756732761171945No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAC3310.1718560977762548No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTACACTGACGACAGG3030.15731842183143566No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTACTCGCGAATCT2940.1526455974206009No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGTA2940.1526455974206009No Hit
TCTGTATCAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGT2900.15056878657134107No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2860.14849197572208117No Hit
ATACGGCCAGGCATTGAAGTCCTTTTCGCAGAGCAGTCAGAGGACCAGGA2810.14589596216050632No Hit
ATACGGCCAGGCATTGAAGTACACTGGCGACAGGGGCCAGGAGGAGACCA2790.14485755673587639No Hit
CTCTCAGCAGGCAAAGACCAGACCAAGTCTCTGCTACCGTAGTACTCGCG2650.13758871876346682No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT2630.13655031333883688No Hit
CAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTAC2580.13395429977726203No Hit
ATACGGCCAGGCATTGAAGTGAAAGAGACAGGGGCCAGGAGGAGACCAAG2570.13343509706494708No Hit
GGGGTCAGAGGCAAGCATTATGAGGAGATAACACAGGCCCAAGAGACCAA2540.13187748892800216No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTAGTACTCGC2530.13135828621568718No Hit
ATACGGCCAGGCATTGAAGTTCACTGACGACAGGGGCCAGGAGGAGACCA2520.13083908350337223No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTACTCGC2520.13083908350337223No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCACCGTAGCTACCTGTTAAAGAA2420.12564705638022253No Hit
ATACGGCCAGGCATTGAAGTCTCTGCTACCGTAGACAGGGGCCAGGAGGA2420.12564705638022253No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACATGGTTCTACAGGGGCCAGG2390.12408944824327763No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCGAG2310.11993582654475786No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAT2280.11837821840781297No Hit
CAGAGGGGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAATCTCG2260.11733981298318302No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAA2250.11682061027086806No Hit
ATACGGCCAGGCATTGAAGTAACACTGACGACAGGGGCCAGGAGGAGACC2230.11578220484623812No Hit
ATACGGCCCGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA2230.11578220484623812No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG2220.11526300213392315No Hit
GCAACAGAAGAGGGGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAT2190.11370539399697824No Hit
GCAACAGAAGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA2160.11214778586003332No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA2120.11007097501077347No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAGTACTC2120.11007097501077347No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG2100.10903256958614352No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTACTCGCGAAT2050.10643655602456867No Hit
GGATGCAGAGCTTCTTCCCATCGCTATCTGAGCAGCGCTCATGCTGGGTG2050.10643655602456867No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTACTCGC2030.10539815059993875No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGATGGGAGGCTG1960.10176373161373395No Hit
GAGAAAGCCCCCCTACTGCTACCGTACTTCCACTCGGATAAGATGCTAGA1960.10176373161373395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)