FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192603
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT95294.947482645649341No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG88124.575214300919508No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA84734.399204581444733No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC64033.3244549669527474No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACGGTTGGAAAG55672.890401499457433No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA45302.3519882867868103No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA36371.8883402646895426No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA36061.8722449806077786No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA34611.7969605873221082No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA32471.6858512068867046No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA30611.5892795023961206No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG30001.5576081369449075No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT29881.5513777043971277No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG29691.5415128528631434No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA29461.5295711904798992No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG27281.4163849991952357No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT26461.3738103767854084No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG23791.2351832525973117No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT23371.213376738680083No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT22891.1884550084889642No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG20951.0877296822998603No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT20521.0654039656703167No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT19541.0145220998634497No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC18790.9755818964398271Illumina Single End PCR Primer 1 (100% over 21bp)
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA17880.9283344496191648No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT16530.8582420834566441No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14450.7502479192951303No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA14430.7492095138705005No Hit
AGAATGGTCCTGCACCAGTAACATGCATATTAAAACAAGATTTACCTCTA12630.655753025653806No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA12510.6495225931060264No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT10840.5628157401494266No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA10180.5285483611366386No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA9790.5082994553563548No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC9600.49843460382237037No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC9130.4740320763435668No Hit
AAGACCCAGGTCCAGATGAACCATGTCGTCAGTGTACTACAGGGCAGCTA8110.42107339968743995No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC7910.41068934544114055No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7860.40809333187956576No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA7820.4060165210303059No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG7550.3919980477978017No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG7090.3681147230313131No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7020.36448030404510834No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG6830.3546154525111239No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC6730.3494234253879742No Hit
CTTGGGCCTGTGTTATCTCCTCTTCCCTGCTTGCCTCTGACCCCTGTAGA6470.33592415486778504No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG5860.3042527894165719No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC5830.30269518127962697No Hit
GGTCTTTGCCTGCTGAGAGTGTAGAACCATGTCGTCAGTGTACTCCAAAG5770.2995799650057372No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT5770.2995799650057372No Hit
CCTGGTCCTCTGACTGCTCTCTCAAAGGGAATGTTCAACTCTGTGACTTC5350.2777734510885085No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5320.2762158429515636No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT5050.2621973697190594No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGCGGTTGGAAAG4930.2559669371712798No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA4730.2455828829249804No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA4410.2289683961309014No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGACTTCAATGCCTGGCCG4110.21339231476145232No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG4000.20768108492598766No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGTGCCTCTG3950.2050850713644128No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC3640.18898978728264876No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG3210.1666640706531051No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC3030.15731842183143566No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG3030.15731842183143566No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2950.1531648001329159No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACCATGTCGTCAGTGTACTTCAATGC2920.151607191995971No Hit
GCCTCAAAGACAATGGCTGATACAGATGTAGAACCATGTCGTCAGTGTAG2920.151607191995971No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA2890.15004958385902606No Hit
CCAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT2890.15004958385902606No Hit
CCTCCTGGCCCCTGTCGCCAGTGTACTTCAATGCCTGGCCGTATTGTAGA2860.14849197572208117No Hit
CCTGGTCCTCTGACTGCTCTGCGAAAAGGACTTCAATGCCTGGCCGTATT2800.14537675944819134No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACGGTCTA2760.1432999485989315No Hit
CCTCCTGGCCCCTGTCTCTTTCACTTCAATGCCTGGCCGTATTGTAGAAC2640.13706951605115184No Hit
CCTCCTGGCCCCTGTCTACGGTAGCAGAGACTTCAATGCCTGGCCGTATT2610.13551190791420695No Hit
CCTCCTGGCCCCTGTCGTCAGTGAACTTCAATGCCTGGCCGTATTGTAGA2570.13343509706494708No Hit
CCTGGTCCTCTGACTGCTCTGTTGTGTAGAACCATGTCGTCAGTGTAGAT2560.1329158943526321No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2530.13135828621568718No Hit
CTTGGGCCTGTGTTATCTCCTCATAATGCTTGCCTCTGACCCCTGTAGAA2510.13031988079105727No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT2510.13031988079105727No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG2510.13031988079105727No Hit
GGTCTTTGCCTGCTGAGAGTGTAGAACCATGTCGTCAGTGTAGATCTCGG2400.12460865095559259No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2390.12408944824327763No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGACTTCAATGCCTGGCCG2340.12149343468170277No Hit
CCCCCCCCTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2320.12045502925707285Illumina Single End PCR Primer 1 (95% over 22bp)
CGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG2270.117859015695498No Hit
CCTGGTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2260.11733981298318302No Hit
CCTCCTGGCCCCTGTCGTCAGTGTTACTTCAATGCCTGGCCGTATTGTAG2250.11682061027086806No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2160.11214778586003332Illumina Single End PCR Primer 1 (95% over 22bp)
TCCAGATGATTCTTTAACAGGTAGCTACGGTGCAGAGACTGGGTCGTAGC2150.11162858314771835No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC2140.1111093804354034No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA2120.11007097501077347No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGTGGGGGTTGTC2030.10539815059993875No Hit
CCTCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2010.10435974517530879No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT2000.10384054246299383No Hit
GCCTCAAAGACAATGGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG1970.10228293432604892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)