FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245778
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA96263.916542570938001No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA82703.364825167427516No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTGAGCGCAAT76653.1186680663037376No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA72412.9461546598963295No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT64842.638153130060461No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC64732.6336775464036655No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA61832.5156848863608623No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC61362.4965619380090978No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG58732.389554801487521No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA47811.9452514057401393No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT46691.8996818266891262No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC46131.8768970371636193No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG42631.734492102629202No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG38491.5660474086370626No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT37401.5216984433106298No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG36701.4932174564037466No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT34291.395161487195762No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG30261.2311923768604187No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT30201.2287511494112573No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG29971.2193931108561384No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT23690.9638779711772412No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA23480.9553336751051762No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA23310.9484168639992189No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC23120.9406863104102076No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG22200.9032541561897321No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA21310.8670426156938376No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT20090.8174043242275549No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA19820.8064188007063284No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG18900.7689866464858531No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA18870.7677660327612723No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC18770.7636973203460032No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA17610.7165002563288823No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC16110.6554695700998462No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG15740.6404153341633506No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC15590.6343122655404471No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCTGAGCGCAATCTCG15250.6204786433285323No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG14740.59972821001066No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC11650.4740049963788459No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA10720.4361659709168436No Hit
CAGAGGGGAAAAATATGACAAAGAATATCGTGCTAAAATGGAGATTCTCT10670.43413161470920913No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCTAATTCAGGACCCA10470.425994189878671No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG10130.4121605676667562No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT10060.4093124689760678No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA9870.40158191538705656No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG9590.39018952062430323No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC8920.3629291474420005No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA8750.35601233633604307No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA8400.3417718428826014No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATCT7350.2990503625222762No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTGAGCGCAAT7250.2949816501070071No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATCTGAGCGCAATCT6960.28318238410272684No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG6650.27056937561539274No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT6480.26365256450943536No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT6210.25266704098820886No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA6090.247784586089886No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGTCCCTGCTACCGAAAG5740.2335440926364443No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC5710.23232347891186356No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT5630.22906850897964828No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA5170.2103524318694106No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGTCTCTGCTACCGAAAG4940.20099439331429178No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC4930.20058752207276487No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT4710.1916363547591729No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4590.18675389986085003No Hit
GGAATCCTATGGCTTTCCAACCATGGTTCTACAGCAACAGAGGAGGGGGA4430.18024395999641954No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTTTGTAACACC4330.17617524758115047No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG4250.17292027764893522No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA4230.1721065351658814No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT3940.16030726916160112No Hit
TACCCTCCATGAGGCACACCAAGTCTCTGCTACCGTAAGCCAGAATTTAC3910.1590866554370204No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCT3890.1582729129539666No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACCAAGGGAGATAACACAGGCCC3660.14891487439884774No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACACTCTCAGCAGGCAAAGAC3620.1472873894327401No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGTCTCTGCTACCGTATC3570.1452530332251056No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT3310.134674380945406No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCTGAGCGCAAC3160.1285713123225024No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGCGGAT3160.1285713123225024No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT3100.126130084873341No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG2960.12043388749196429No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCTGAGCGCAA2950.1200270162504374No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATCT2840.11555143259364142No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATC2700.10985523521226473No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCTGAGCG2650.10782087900463021No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC2650.10782087900463021No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATC2620.10660026528004948No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCCGGTGGC2590.10537965155546876No Hit
GAGAAAGCCCCCCTACTGCACGACATGGTTCTACAGAGGAAAGCCCCCCT2580.10497278031394186No Hit
TGAGCAAGAGGCTTTGGAGTACACTGACGACATGGTTCTACTCTCAGCAG2540.10334529534783424No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACG2540.10334529534783424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)