FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222632
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA103574.652071580006468No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA96864.350677350964821No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT93384.194365589852312No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA84433.792356893887671No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC74453.3440835100075463No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC73893.318929893276797No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA71613.216518739444464No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGTACGTGAT65862.9582449962269575No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT51862.329404577958245No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG47772.145693341478314No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT45382.0383412986452982No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC44602.0033059039131844No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT36211.6264508246792913No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA34801.5631176111250853No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG33801.518200438391606No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC33731.5150562363002624No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG31331.4072550217399116No Hit
TGTGGTCTGCCAGCTAAAGGTTTGTACTTTACTTTCATTGGGAGAAGACC30471.3686262531891191No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC29861.3412267778216969No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT27901.2531891192640772No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA26971.2114161486219412No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG24981.122030974882317No Hit
GGGGTCAGAGGCAAGCAGACGGAGATAACACAGGCCCAAGAGACCAAGTC24181.0860972366955335No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA23901.0735204283301591No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG22431.0074921844119444No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG20680.9288871321283553No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT19000.8534262819361099No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT18070.811653311293974No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC14770.6634266412734917No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC14670.6589349240001438No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA13400.6018901146286249No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATATGTACGTGATCTCG12900.579431528261885No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA12870.5780840130798808No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT11800.5300226382550577No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCGTAGATGCCGACGAGTACC11110.49902978906895684No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG10940.49139386970426535No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATATGTAC10570.474774515792878No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATAGATTCCTCCAATTCAGGACCCA10190.4577059901541557No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC8880.39886449387329764No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGGGAGGTGGCTTTAGGTCAGC8770.39392360487261485No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA8620.387186028962593No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAT8560.3844909985985842No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATATGTACGTGATCT8270.37146501850587516No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT8050.3615832405045097No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG7200.3234036436810521No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA6840.30723346149699954No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG6800.3054367745876604No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG6400.28746990549426854No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG6130.2753422688562291No Hit
TGAGCAAGAGGCTTTGGAGTCAGAGACTCTCAGCAGGCAAAGACCAGACC6010.2699522081282116No Hit
GGAATCCTATGGCTTTCCAACCACTGACGACATGGTTCTACAGCAACAGA5820.2614179453088505No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC5070.22773006575874088No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATAT4800.2156024291207014No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGTACGTGAT4590.2061698228466707No Hit
ATACGGCCAGGCATTGAAGTCACACTGACGACAGGGGCCAGGAGGAGACC4530.20347479248266198No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATAT3800.17068525638722196No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA3630.16304933702253047No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCCTCATACCAGGACCA3520.1581084480218477No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATATGTACGTGA3430.15406590247583454No Hit
GGAAACCGTAGCTGCCCTGTAGAACTTCATCTGGACCTGGGTCTTAGACC3230.14508246792913868No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC3070.13789572029178196No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATAAC3000.13475151820043837No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTAGACCTGGGTCTTCAGTGAAC2910.13070897265442524No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA2870.12891228574508606No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTATATGTACG2850.12801394229041646No Hit
ATACGGCCAGGCATTGAAGTCCCATGGAAGCCAGCCCCTCAGGGCAACTG2840.1275647705630817No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGTACGTGAC2790.1253189119264077No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATA2690.12082719465305976No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA2690.12082719465305976No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTATATGTACGTGATCT2660.11947967947105538No Hit
ATACGGCCCGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA2650.11903050774372058No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2600.11678464910704661No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATATGTA2520.11319127528836825No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA2480.11139458837902906No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCGAG2330.10465701246900716No Hit
AGGACAAACTGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA2320.10420784074167234No Hit
TGTGGTCTGCCAGCTAAAGGTTTGTACTTTACTTTCATTGGGAGGAGACC2260.10151281037766359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)