FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222632
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA104094.675428509827877No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC88523.976068130367602No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA81263.649969456322541No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG73783.313989004276115No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT71503.2115778504437817No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA67673.0395450788745553No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT56462.5360235725322506No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC48402.173991160300406No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG47412.129523159294261No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA46532.0899960472887993No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG46062.0688849761040644No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC42831.9238025081749255No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA31731.4252218908333034No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT31631.4207301735599553No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTAGCTGGCAGACCACATGTAG31191.4009666175572244No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG31091.3964749002838766No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA29241.3133781307269394No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA28891.2976571202702216No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA28571.2832836249955082No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC28131.2635200689927775No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC26341.183118329799849No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG24591.10451327751626No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC24321.0923856408782204No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG23641.0618419634194545No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT23541.0573502461461066No Hit
CTTGGGCCTGTGTTATCTCCGTCTGCTTGCCTCTGACCCCTGTAGAACCA23531.056901074418772No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG21860.981889395953861No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG19160.8606130295734666No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT16680.7492184411944375No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA16280.7312515721010456No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC15870.7128355312803191No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT14830.6661216716375005No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC14760.6629774695461569Illumina Single End PCR Primer 1 (100% over 21bp)
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA13800.6198569837220166No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC12890.5789823565345502No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT11290.5071148801609832No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC10620.4770203744295519No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT9880.4437816666067771No Hit
ATGAGGTACTCGTCGGCATCTACGGTAGCAGAGACTTGGCTATAGGAGCA9380.4213230802400374No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8880.39886449387329764No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC8820.3961694635092889No Hit
GCTGACCTAAAGCCACCTCCCTTCTACCATGCCCTGAAACAGAGAAGACC8660.38898271587193217No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC8410.3777534226885623No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8150.36607495777785765No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT7510.3373279672284308No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA7000.31442020913435625No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT6970.3130726939523519No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA6970.3130726939523519No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC6740.3027417442236516No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA6410.28791907722160337No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC6300.28297818822092063No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC6250.28073232958424665No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGTTGGAAAGCCAT5830.26186711703618526No Hit
GGTCTTTGCCTGCTGAGAGTCTCTGACTCCAAAGCCTCTTGCTCATGTAG5790.2600704301268461No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT5720.2569262280355025No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5160.23177261130475404No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA4920.22099248984871894No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC4630.2079665097560099No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG4590.2061698228466707No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA4570.20527147939200116No Hit
CCTCCTGGCCCCTGTCGTCAGTGTGACTTCAATGCCTGGCCGTATTGTAG4530.20347479248266198No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG4190.18820295375327895No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA3890.17472780193323512No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT3500.15721010456717813No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG3450.15496424593050415No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG3390.15226921556649536No Hit
AAGACCCAGGTCCAGATGAAGTTCTACAGGGCAGCTACGGTTTCCTGTAG3080.13834489201911673No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG2840.1275647705630817No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2790.1253189119264077No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG2610.1172338208343814No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG2600.11678464910704661No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATATTGCAAATGCTATC2570.11543713392504222No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACT2460.11049624492435947No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTAGCTGGCAGACCACATGTAG2400.1078012145603507No Hit
CGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG2390.10735204283301591No Hit
GCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2340.10510618419634193No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT2320.10420784074167234No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAAACCAAAATCAC2310.10375866901433757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)