FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195220
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA97705.00461018338285No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA82084.204487245159307No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT81094.153775227947956No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA76853.936584366355906No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTATACAGTGAT74843.83362360413892No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA73653.7726667349656795No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC65513.355701260116791No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA49972.5596762626780043No Hit
GTAGAGGAGCCGTCAAATCCAGAAGACCAAGTCTCTGCTACCGTACTATA37591.9255199262370657No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA35391.8128265546562852No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC32711.6755455383669706No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA30131.5433869480586007No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG29281.4998463272205715No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT28511.4604036471672985No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC28361.4527200081958815No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA25151.2882901342075608No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT24111.2350169040057373No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT22991.1776457330191579No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC21691.1110541952668784No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG20291.0393402315336542No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC19030.9747976641737526No Hit
GGGGTCAGAGGCAAGCAGGGATGGGAGATAACACAGGCCCAAGAGACCAA18760.9609671140252024No Hit
TCCACAAACAGAACAAGATGCTACGTAATCCTGTTTGAAGGAAAAGGCCA16470.8436635590615715No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACTATACAGTGATCTCG15800.8093433049892429No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT13050.668476590513267No Hit
GGAATCCTATGGCTTTCCAACCCTGACGACATGGTTCTACAGCAACAGAG12840.6577194959532835No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC12250.6274971826657104No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA11300.578834135846737No Hit
GAGAAAGCCCCCCTACTGCCATGGTTCTACATACCTTCCACTCGGATAAG10290.5270976334391968No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG9650.49431410716115154No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTA9400.48150804220879007No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTA9350.47894682921831777No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG8440.4323327527917221No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACACTGACGAC8150.41747771744698287No Hit
GGAATCCTATGGCTTTCCAACCCGACATGGTTCTACAGCAACAGAGGAGG8010.4103063210736605No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCACCGTAGCTACCTGTTAAAGAA7930.4062083802889048No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG7850.4021104395041492No Hit
GGAATCCTATGGCTTTCCAACACTGACGACATGGTTCTACAGCAACAGAG7690.39391455793463787No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACTATACAGTGATCT7650.39186558754226No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA7600.38930437455178774No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC7570.38776764675750436No Hit
GTAGAGGAGCCGTCAAATCCAGAAGACCAAGTCTCTGCTACCGTATCAAG7420.38008400778608753No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACT6630.33961684253662533No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTATACAGTGA6470.33142096096711404No Hit
GAGAAAGCCCCCCTACTGCTACACTGACGACATGGTTCTACAGAGAAAGC6430.32937199057473615No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC6370.32629853498616945No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA6300.32271283679950824No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGAAGTCTCTTCATCTGGACCTGGG6260.3206638664071304No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA5930.3037598606700133No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG5910.3027353754738244No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG5900.30222313287572994No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTATACAGTGAT5730.29351500870812414No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG5620.28788034012908514No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC5170.26482942321483455No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTACTA4600.23563159512345047No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT4190.21462964860157768No Hit
ATACGGCCAGGCATTGAAGTGACAGGGGCCAGGAGGAGACCAAGTCTCTG3890.19926237065874397No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGGAGACCA3790.1941399446777994No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3500.17928490933306013No Hit
GGTCTTCTCTGTTTCAGGGCATCCTGCCAGCCCTGCCAGGCCCACAGCAG3430.17569921114639891No Hit
AGAAAACGGCATTTTGAGTGTGGCATAACTGCACCCTTAGACCAAGTCTC3380.17313799815592665No Hit
GGATGCAGAGCTTCTTCCCACAGGTCTCTGCTACCGTACTGGGTGCGGAA3310.16955229996926544No Hit
CAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTAT3300.169040057371171No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG3270.16750332957688763No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTACTATACAG3260.16699108697879314No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA3240.16596660178260425No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTATACAGTGAC3190.16340538879213196No Hit
GCGTGGACAACCCCCACGACACTGACGACATGGTTCTACAGCAACAGAGG3080.1577707202130929No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTACTATAC3030.15520950722262064No Hit
ATACGGCCAGGCATTGAAGTGTACACTGACGACAGGGGCCAGGAGGAGAC2990.1531605368302428No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACT2900.1485503534473927No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA2790.14291568486835365No Hit
TGAGCAAGAGGCTTTGGAGTCAGTGTCACTCTCAGCAGGCAAAGACCAGA2690.13779325888740906No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTACTATACAGTGATCT2640.1352320458969368No Hit
ATACGGCCCGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA2610.13369531810265342No Hit
CAGAGGGGGGGGAGACCAAGTCTCTGCTACCGTACTATACAGTGATCTCG2490.12754840692551994No Hit
GGGGTCAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACC2490.12754840692551994No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACTATACAG2470.12652392172933102No Hit
CTGACGACAGGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACTATACA2460.12601167913123656No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA2420.12396270873885873No Hit
GCAACAGAAGAGGGGGAGACCAAGTCTCTGCTACCGTACTATACAGTGAT2360.12088925315029199No Hit
GCAACAGAAGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA2240.11474234197315848No Hit
GGTCTTCTCTGTTTCAGGGCACACACTGACGACATGGTTCTACAGCAACA2180.11166888638459173No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC2150.11013215859030838No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTACTATACAG2090.10705870300174163No Hit
AGGACAAACTGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA2080.10654646040364715No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG2050.1050097326093638No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTATACAGTGAT2040.10449749001126933No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTACTATACAGTGATCTCG1970.10091179182460813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)