FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195220
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT105585.408257350681283No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA98975.069664993340846No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG83794.29208072943346No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC75783.881774408359799No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA75173.8505276098760373No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA60083.077553529351501No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA48612.490011269337158No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA47962.4567155004610184No Hit
TCTGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGATC37271.9091281630980432No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA31291.6028070894375575No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA30931.5843663559061572No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG28391.454256735990165No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA26691.3671754943141072No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG25791.321073660485606No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG25041.2826554656285216No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG24391.249359696752382No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC22771.1663763958610798No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG19591.0034832496670423No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG18610.9532834750537854No Hit
CTTGGGCCTGTGTTATCTCCCATCCCTGCTTGCCTCTGACCCCTGTAGAA18540.9496977768671243No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC17480.8954000614691118Illumina Single End PCR Primer 1 (100% over 21bp)
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA16080.8236860977358876No Hit
GGCCTTTTCCTTCAAACAGGATTACGTAGCATCTTGTTCTGTTTGTGGAT15460.7919270566540314No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA14930.7647781989550251No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA14660.7509476488064748No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGGTTGGAAAGCCATA13280.6802581702694396No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA11810.6049585083495543No Hit
AGCATCTTATCCGAGTGGAAGGTATGTAGAACCATGGCAGTAGGGGGGCT11390.5834443192295871No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTAGCAGAGACTGGGTCGTAG10130.5189017518696856No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA9360.47945907181641223No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8880.4548714271078783No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC8490.4348939657821944No Hit
CCTCCTGGCCCCTGTCGTCAGTGTAGAACCATGTCGTCAGTGTACTTCAA8390.4297715398012498No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC8260.42311238602602197No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGGGTTGGAAAGCCATAGGAT8230.42157565823173854No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC8150.41747771744698287No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7880.40364716729843253No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTTGGAAAGCCATA7830.40108595430796024No Hit
TCCAGATGATTCTTTAACAGGTAGCTACGGTGCAGAGACTGGGTCGTAGC7430.38059625038418193No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7170.36727794283372606No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG6690.3426902981251921No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT6670.34166581292900317No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG6480.3319332035652085No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG6410.3283475053785473No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT6350.32527404978998053No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT6290.3222005942014138No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG6240.3196393812109415No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA5910.3027353754738244No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC5910.3027353754738244No Hit
AAGACCCAGGTCCAGATGAAGAGACTTCTAAGACCCAGGGCAGCTACGGT5820.29812519209097427No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG5650.2894170679233685No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC5520.28275791414814055No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT4680.23972953590820612No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA4550.2330703821329782No Hit
AGCATCTTATCCGAGTGGAAGGGGGGCTTTCTCTGTAGAACCATGTCGTC4490.22999692654441145No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG4340.22231328757299457No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT4080.20899498002253866No Hit
CCTCCTGGCCCCTGTCACTTCAATGCCTGGCCGTATTGTAGAACCATGTC4020.2059215244339719No Hit
CCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA3760.19260321688351603No Hit
AAGGGTGCAGTTATGCCACACTCAAAATGCCGTTTTCTTGTAGAACCATG3380.17313799815592665No Hit
GCTGACCTAAAGCCACCTCCAGGGACAAAGAGGAGCTGCTGTGGGCCTGG3360.17211351295973773No Hit
CCTGGTCCTCTGACTGCTCTGTTGTGTAGAACCATGTCGTCAGTGTAGAT3200.16391763139022641No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG3180.1628931461940375No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACACTTCAATGCCTGGCCGTATTGTA3000.15367277942833726No Hit
TTCTCTTCCGCACCCAGTACGGTAGCAGAGACCTGTGGGAAGAAGCTCTG2940.1505993238397705No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT2850.14598914045692038No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2840.14547689785882595No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTC2830.1449646552607315No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT2830.1449646552607315No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG2790.14291568486835365No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2680.1372810162893146No Hit
CCCCCCCCTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2590.1326708329064645Illumina Single End PCR Primer 1 (95% over 22bp)
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC2570.13164634771027559No Hit
GGTCTTTGCCTGCTGAGAGTGACACTGACTCCAAAGCCTCTTGCTCATGT2570.13164634771027559No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGACCCCTGTAGAACCATGTCGTCAG2560.13113410511218113No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2420.12396270873885873No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG2410.12345046614076426No Hit
CCTGGTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2340.11986476795410306No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG2270.11627906976744186No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2240.11474234197315848Illumina Single End PCR Primer 1 (95% over 22bp)
CCTCCTGGCCCCTGTCGTCAGTGTAGAACCATGTCGTCAGTGTAGATCTC2220.11371785677696958No Hit
GCCTCAAAGACAATGGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG2090.10705870300174163No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC2040.10449749001126933No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2040.10449749001126933No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA1990.10193627702079705No Hit
CCCCCTCCTCTGTTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1970.10091179182460813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)