FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274296
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA97523.5552833435412836No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAT87453.1881616939364776No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA87213.1794120220491733No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT85573.119622597485928No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC74992.7339078951205997No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC67742.469594890191618No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA67152.448085280135328No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG58082.1174205967276225No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT54461.9854463790941173No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA50731.8494618951789308No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA48471.7670691515734824No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT47881.7455595415171932No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG40631.4812465365882113No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA40611.4805173972642693No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT37711.3747921952926765No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG37501.3671362323912852No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC37441.3649488144194593No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC33271.2129232653775484No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT32201.1739143115466504No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT30701.119228862250999No Hit
AGAAAACGGCATTTTGAGTGTACACTGACGACATGGTTCTACGAAAACGG29711.083136465715869No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC27611.006576836701957No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA27451.000743722110421No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA27230.9927231895470586No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA24410.889914544871234No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT23960.8735089100825386No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG23480.8560095663079301No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG21710.7914807361390614No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG21500.7838247732376702No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC20350.7418992621110042No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATGATACTCTGATCTCG19760.7203896520547146No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA19510.7112754105054394No Hit
GACCCAAAACCCAAAATGGCCAGTAGGAGACAGAAGCAGGGAAGACCAAG19460.7094525621955844No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG17880.6518505556041648No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG17440.6358094904774405No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC16750.6106541838014408No Hit
TGTTCATTCATGATCCCACTGCTACCGTAACAAGTGTGAGTGACTGGTGG16350.5960713973226004No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG15880.578936623209963No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG15020.5475836322804561No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC14000.5103975267594132No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG13590.49545017061860175No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT13200.4812319538017324No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC13070.4764925481961093No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG12820.4673783066468341No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT11860.4323796190976172No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA11660.425088225858197No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA11270.41087000904132764No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGA10770.3926415259427772No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC10020.36529880129495146No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA9770.3561845597456762No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA9140.3332166710415026No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT9040.3295709744217925No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT8000.2916557295768076No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA7350.26795870154869195No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAT6930.2526467757459095No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATGATACTCTGATCT6890.2511884970980255No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAACAGTTCAACTTCTGTAACACC6870.25045935777408346No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG6420.23405372298538804No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCCCCAATTCAGGACCCA6190.2256686207600548No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGGAA6170.2249394814361128No Hit
TCCACAAACAGAACAAGATGCTACATGACGACATGGTTCTAATCCTGTTT5440.19832589611222914No Hit
CAGCCTCTGGCATTCTGGCTTGCAGTTGGCCCTATAGAGAGCAGTCAGAG5250.19139907253477995No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA5160.18811794557704087No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG5130.18702423659112782No Hit
TCCACAAACAGAACAAGATGCTACACTGACGACATGGTTCTAAATCCTGT5100.1859305276052148No Hit
AAAACGGCATTTTGAGGCATAACTGCACCCTTAGACCAAGTCTCTGCTAC4940.18009741301367865No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG4900.17863913436579462No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG4780.1742642984221425No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA4670.17025403214046142No Hit
GACCCAAAACCCAAAATGGCCGGTAGGAGACAGAAGCAGGGAAGACCAAG4400.16041065126724416No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG4390.16004608160527314No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGA4350.1585878029573891No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATGATACT4150.1512964097179689No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT4150.1512964097179689No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAAACATTATGACA4010.14619243445037478No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGATACTC3830.13963018053489662No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAC3820.1392656108729256No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCTGGG3750.13671362323912853No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3730.13598448391518653No Hit
GTAGAGGAGCCGTCAAATCCAGAAGCTAGCAGTTCAACTTCTGTAACACC3670.13379706594336044No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTAGTGCTAAAATGGAGATTC3650.13306792661941844No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAATCTCTGCTACCGTATTC3640.13270335695744742No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATGA3560.12978679966167936No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC3360.12249540642225917No Hit
ACTTCTACCGTGCCCTGAGTTATCAAGCTGTGTCTACTCATTTGAACAGA3050.11119374690115788No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGATACTCTGA3040.11082917723918688No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGCAACACC3030.11046460757721585No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG2930.10681891095750576No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATG2890.10536063230962173No Hit
GCAACAGAAGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAT2800.10207950535188265No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATGATACTCTGAT2780.10135036602794062No Hit
TCACAATTGCCAGTTAACGTCTGTCTTCCCTGTTTCTGCTTTGCTGTGTG2760.1006212267039986No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT2760.1006212267039986No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT2760.1006212267039986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)