FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274296
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG98573.59356315804824No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA87753.1990987837956077No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC84643.085717618922624No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT80362.9296818035990317No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA77462.823956601627439No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA62642.283664362586403No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC54811.9982063172631026No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA52671.9201884096013064No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT47891.7459241111791641No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC45271.6504068597427597No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA42221.5392131128416018No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG41631.5177035027853123No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG40861.4896316388135444No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT40791.4870796511797475No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG37571.3696882200250824No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA37521.3678653717152274No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC36281.3226587336308222No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC29741.084230174701782No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTTTCGTAGAACCATGTCGTCA29351.0700119578849125No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA26900.9806923907020154No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA26840.9785049727301893No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG26410.9628284772654359No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT24290.8855397089275818No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT23550.8585615539417273No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC22900.8348645259136115Illumina Single End PCR Primer 1 (100% over 21bp)
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG21270.775439671012337No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG21070.7681482777729168No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT20740.7561174789278735No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC19900.7254936273223087No Hit
TCCCTGCTTCTGTCTCCTACTGGCCATTTTGGGTTTTGGGTCTGTAGAAC19400.7072651442237583No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA19320.7043485869279902No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT19110.696692624026599No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA17680.6445591623647446No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT16850.6142998804211508No Hit
CCACCAGTCACTCACACTTGTTACGGTAGCAGTGGGATCATGAATGAACA16310.5946131186747164No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC16290.5938839793507743No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA16060.5854988771254411No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC15570.5676349636888617No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC15390.5610727097733835No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG14830.5406568087030069No Hit
GCTGCCCCCACCATGAGCGCTGTTCAGATAGCGATGGTGAGCAGCTGGGG14800.5395630997170939No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT13840.5045644121678771No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT12950.4721177122524572No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT12450.4538892291539067No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC11950.43566074605535626No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT10460.38133986642167583No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT10440.3806107270977338No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA10400.3791524484498498No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC10320.37623589115408174No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG10150.37003820690057454No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT9870.3598302563653863No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT8920.3251961384781404No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8880.3237378598302564No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT8210.2993116924781987No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC8140.2967597048444017No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG8080.29457228687257564No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG7460.27196896783037305No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT7320.2668649925627789No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7190.26212558695715577No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7150.26066730830927176No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA6960.25374048473182254No Hit
CCATGGGACTGACTTTCTGCTCTTTCCACCCATCTACAGTCCCCCTTGCC5950.21691894887275062No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT5500.2005133140840552No Hit
GGCCTTTTCCTTCAAACAGGATTAGAACCATGTCGTCATGTAGCATCTTG5450.19869046577420013No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCTCTTGCCGTC5400.19686761746434506No Hit
CCTGGTCCTCTGACTGCTCTCTATAGGGCCAACTGCAAGCCAGAATGCCA5330.19431562983054804No Hit
GGCCTTTTCCTTCAAACAGGATTTAGAACCATGTCGTCAGTGTAGCATCT5220.1903053635488669No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG4830.17608714673199755No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAGT4800.17499343774608453No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA4710.17171231078834545No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTGAT4510.16442091754892524No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT4510.16442091754892524No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA4470.1629626389010412No Hit
TCCCTGCTTCTGTCTCCTACCGGCCATTTTGGGTTTTGGGTCTGTAGAAC4310.15712952430950505No Hit
AAGGGTGCAGTTATGCCTCAAAATGCCGTTTTTGTAGAACCATGTCGTCA4300.15676495464753404No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG4140.1509318400559979No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG4070.14837985242220084No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC4040.14728614343628782No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC3880.14145302884475164No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG3720.1356199142532155No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA3590.13088050864759238No Hit
AAGACCCAGGTCCAGATGAATACGGTAGCAGAGATTTGGTCTAAGACCCA3580.13051593898562136No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC3500.1275993816898533No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA3490.12723481202788228No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTTGTCATATTTTTCC3480.12687024236591127No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA3420.12468282439408523No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT3400.1239536850701432No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA3370.12285997608423019No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA3320.12103712777437511No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG3240.11812057047860705No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAAATACAGTCTA3220.11739143115466504No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT3140.11447487385889696No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3100.11301659521101293No Hit
GTTCAAATGAGTAGACACAGCTTGATAACTCAGGGCACGGTAGAAGTTGT3070.1119228862250999No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2930.10681891095750576Illumina Single End PCR Primer 1 (95% over 22bp)
CCACACAGCAAAGCAGAAACAGGGAAGACAGACGTTAACTGGCAATTGTG2910.10608977163356374No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT2880.10499606264765071No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG2830.10317321433779567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)