FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278681
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA145085.205952325418669No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA126014.521657378866877No Hit
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG115244.1351940031792624No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC113374.068092191430345No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC105793.7960966122555897No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT100703.6134505043400877No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA98353.5291246981315556No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT90763.2567702857388916No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG90493.2470817888553576No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT88823.1871566414646133No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC88283.1677796476975466No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA85393.0640768477219473No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT84193.0210168615729094No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC83102.981904040820867No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG82962.9768803757701456No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG82072.9449442193762763No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG81182.9130080629824064No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA73322.630965153706209No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG72642.606564494888421No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT66982.403464893552126No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG64922.3295452506629446No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG58292.0916388271895103No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCCCTC51751.8569619026772546No Hit
TGACAGGGGCCAGGAGTTTGGGTCTTCAGTGAACCATTGAGACCAAGTCT11680.4191171985173011No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC9780.350938887114658No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC7720.2770192442254764No Hit
TGACAGGGGCCAGGAGGACTCACTGCCCTGAAGGAAAGGACATAAGCCTG7530.2702014130852121No Hit
TGACAGGGGCCAGGAGAATTGCTTGTACCTGGGAAGCAGAGGTTGCAGTT6510.23360042485853No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGGTCTTCAGTGAACCATTGA5590.20058776881093435No Hit
TGACAGGGGCCAGGAGCTCAGCCTGTGGGGTGGGTCTTCAGTGAACCATT4580.1643456138021609No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGTTCTACATGACATGGGTCT3750.1345624567157431No Hit
AGGAAGGGGCTGAGGTCACTTACCTGGAGTGAGCCCTGCTCCCCCCTGGC3420.1227209605247577No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGTTCTACATGACAGGGGCCA3340.11985029478148851No Hit
TGACAGGGGCCAGGAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG3090.11087946433377231No Hit
TGACAGGGGCCAGGAGCGTGATTAAGTTAACCATGGGTCTTCAGTGAACC2950.10585579928305124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)