FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278681
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG141565.0796430327148245No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT121394.355876432193081No Hit
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT118034.235308470975776No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA107793.867863255837319No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA103693.720741636494774No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT102353.672657985295015No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA99803.5811555147283096No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG89633.2162221321152145No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA88893.189668473989974No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC86533.104983834563533No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC86203.0931423383725476No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA85643.0730476781696634No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC84533.0332171909818033No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC81032.9076255647137765No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC79422.8498534166304843No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC78152.804281597956086No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT75112.695196299711857No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA67712.4296597184594573No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA67382.4178182222684717No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA58922.1142453199177553No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT50661.817849081925212No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGCAGGTTGAGGGCTGCTCAG49531.7773009283015346No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG32441.1640549588956548No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG24420.8762707181329189No Hit
CAATGGTTCACTGAAGACCCAAACTCCTGGCCCCTGTCATGTAGAACCAT12010.4309586947082865No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG9490.3405327237953072No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT7430.2666130809061256No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC7120.25548925115095755No Hit
CAATGGTTCACTGAAGACCCAGGCTGGCCTGCAGTGGCACGATCTCAGCT6920.2483125867927846No Hit
CAATGGTTCACTGAAGACCCAAGAATCCCTACAGTTAGCAGGTGGCATAT5830.20919976604074192No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC5240.18802860618413167No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA4720.16936927885288197No Hit
CAATGGTTCACTGAAGACCCACCCCACAGGCTGAGCTCCTGGCCCCTGTC4630.16613977989170411No Hit
CAATGGTTCACTGAAGACCCATGTCATGTAGAACCATGTCGTCAGTGTCT3940.14138028785600742No Hit
CAATGGTTCACTGAAGACCCAGGTCAGGAGCCACTTGCCACCCTGCACAC3780.13563895636946902No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA3340.11985029478148851No Hit
CAATGGTTCACTGAAGACCCATGGTTAACTTAATCACGCTCCTGGCCCCT3050.10944413146213772No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG2950.10585579928305124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)