FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308011
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA159695.184555097058222No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA141564.595939755398346No Hit
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG133974.349519984675871No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC126344.10180155903523No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT126124.0946589569853025No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC125074.060569265383379No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA119943.8940167721282686No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG110703.5940274860313433No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT102883.3401404495293994No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC102113.3151413423546563No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG102103.314816678625114No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT100873.2748830398914324No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG99773.2391700296417985No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG99583.2330014187804985No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA99363.2258588167305717No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC93863.0472937654824017No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT87162.8297690666891766No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG84912.7567197275421984No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG78862.5602981711692117No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT78692.5547788877669952No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG77502.516143903951482No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA68482.223297219904484No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC67392.187908873384392No Hit
TGACAGGGGCCAGGAGTTTGGGTCTTCAGTGAACCATTGAGACCAAGTCT14560.47271039021333655No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC13120.42595881315927026No Hit
GGCCACTGACAACCACCACCACACTATGTCGAAGACCAAGTCTCTGCTAC9010.29252202031745617No Hit
TGACAGGGGCCAGGAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG8840.28700273691524003No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGTTCTACATGACATGGGTCT6430.20875877809558746No Hit
TGACAGGGGCCAGGAGCGGAATGATATAGTTTGGTTCTGTGTTTCCACCC5960.19349958280710755No Hit
TGACAGGGGCCAGGAGTCAGAGAGTCTCCTCTTGTCTATAAATTATGTCT4990.16200720104152125No Hit
TGACAGGGGCCAGGAGTAGTGGCAAAGTCAGCTGCAGCAGGGTGTTATTG4100.13311212911227197No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGGTCTTCAGTGAACCATTGA3660.11882692501241837No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAGATGGGTGAAAAGAGCAGTCA3550.11525562398745501No Hit
TGACAGGGGCCAGGAGGTTGGGTCTTCAGTGAACCATTGAGACCAAGTCT3280.10648970328981756No Hit
TGACAGGGGCCAGGAGGTTGAGGCTACAGTGAATTGAGGCTGCAAAGAGC3240.10519104837164905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)